Microtubule-like properties of the bacterial actin homolog ParM-R1

In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent...

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Main Authors: Popp, David., Narita, Akihiro., Lee, Lin Jie., Larsson, Mårten., Robinson, Robert C.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/104888
http://hdl.handle.net/10220/17016
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1048882022-02-16T16:27:19Z Microtubule-like properties of the bacterial actin homolog ParM-R1 Popp, David. Narita, Akihiro. Lee, Lin Jie. Larsson, Mårten. Robinson, Robert C. School of Biological Sciences DRNTU::Science::Biological sciences::Biochemistry In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent cycles of polymerization and depolymerization. Here, we show that actin homolog, ParM-R1 that drives segregation of the R1 multidrug resistance plasmid from Escherichia coli, can also self-organize in vitro into asters, which resemble astral microtubules. ParM-R1 asters grow from centrosome-like structures consisting of interconnected nodes related by a pseudo 8-fold symmetry. In addition, we show that ParM-R1 is able to perform persistent microtubule-like oscillations of assembly and disassembly. In vitro, a whole population of ParM-R1 filaments is synchronized between phases of growth and shrinkage, leading to prolonged synchronous oscillations even at physiological ParM-R1 concentrations. These results imply that the selection pressure to reliably segregate DNA during cell division has led to common mechanisms within diverse segregation machineries. 2013-10-30T02:14:07Z 2019-12-06T21:42:00Z 2013-10-30T02:14:07Z 2019-12-06T21:42:00Z 2012 2012 Journal Article Popp, D., Narita, A., Lee, L. J., Larsson, M., & Robinson, R. C. (2012). Microtubule-like properties of the bacterial actin homolog ParM-R1. Journal of biological chemistry, 287(44), 37078-37088. https://hdl.handle.net/10356/104888 http://hdl.handle.net/10220/17016 10.1074/jbc.M111.319491 22908230 en Journal of biological chemistry
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Biochemistry
spellingShingle DRNTU::Science::Biological sciences::Biochemistry
Popp, David.
Narita, Akihiro.
Lee, Lin Jie.
Larsson, Mårten.
Robinson, Robert C.
Microtubule-like properties of the bacterial actin homolog ParM-R1
description In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent cycles of polymerization and depolymerization. Here, we show that actin homolog, ParM-R1 that drives segregation of the R1 multidrug resistance plasmid from Escherichia coli, can also self-organize in vitro into asters, which resemble astral microtubules. ParM-R1 asters grow from centrosome-like structures consisting of interconnected nodes related by a pseudo 8-fold symmetry. In addition, we show that ParM-R1 is able to perform persistent microtubule-like oscillations of assembly and disassembly. In vitro, a whole population of ParM-R1 filaments is synchronized between phases of growth and shrinkage, leading to prolonged synchronous oscillations even at physiological ParM-R1 concentrations. These results imply that the selection pressure to reliably segregate DNA during cell division has led to common mechanisms within diverse segregation machineries.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Popp, David.
Narita, Akihiro.
Lee, Lin Jie.
Larsson, Mårten.
Robinson, Robert C.
format Article
author Popp, David.
Narita, Akihiro.
Lee, Lin Jie.
Larsson, Mårten.
Robinson, Robert C.
author_sort Popp, David.
title Microtubule-like properties of the bacterial actin homolog ParM-R1
title_short Microtubule-like properties of the bacterial actin homolog ParM-R1
title_full Microtubule-like properties of the bacterial actin homolog ParM-R1
title_fullStr Microtubule-like properties of the bacterial actin homolog ParM-R1
title_full_unstemmed Microtubule-like properties of the bacterial actin homolog ParM-R1
title_sort microtubule-like properties of the bacterial actin homolog parm-r1
publishDate 2013
url https://hdl.handle.net/10356/104888
http://hdl.handle.net/10220/17016
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