Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector

• Main Authors: Dighe, Niraja, Khoury, Maroun, Mattar, Citra, Chong, Mark, Choolani, Mahesh, Chen, Jianzhu, Antoniou, Michael N., Chan, Jerry Kok Yen
• Other Authors: Santiago, Mario L.
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Chemical engineering::Biochemical engineering
• Online Access: https://hdl.handle.net/10356/104904; http://hdl.handle.net/10220/20409
• Institution: Nanyang Technological University

Hematopoietic Stem Cell (HSC) targeted gene transfer is an attractive treatment option for a number of hematopoietic disorders caused by single gene defects. However, extensive methylation of promoter sequences results in silencing of therapeutic gene expression. The choice of an appropriate promote...