Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins

Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) prese...

Full description

Saved in:
Bibliographic Details
Main Authors: Tjin, Monica Suryana, Chua, Alvin Wen Choong, Ma, Dong Rui, Lee, Seng Teik, Fong, Eileen
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
Subjects:
Online Access:https://hdl.handle.net/10356/105284
http://hdl.handle.net/10220/20681
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-105284
record_format dspace
spelling sg-ntu-dr.10356-1052842020-06-01T10:13:43Z Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins Tjin, Monica Suryana Chua, Alvin Wen Choong Ma, Dong Rui Lee, Seng Teik Fong, Eileen School of Materials Science & Engineering DRNTU::Science::Biological sciences Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering. 2014-09-15T02:46:06Z 2019-12-06T21:48:46Z 2014-09-15T02:46:06Z 2019-12-06T21:48:46Z 2014 2014 Journal Article Tjin, M. S., Chua, A. W. C., Ma, D. R., Lee, S. T., & Fong, E. (2014). Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins. Macromolecular bioscience, 14(8), 1125-1134. 1616-5187 https://hdl.handle.net/10356/105284 http://hdl.handle.net/10220/20681 10.1002/mabi.201400015 en Macromolecular bioscience © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Tjin, Monica Suryana
Chua, Alvin Wen Choong
Ma, Dong Rui
Lee, Seng Teik
Fong, Eileen
Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
description Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Tjin, Monica Suryana
Chua, Alvin Wen Choong
Ma, Dong Rui
Lee, Seng Teik
Fong, Eileen
format Article
author Tjin, Monica Suryana
Chua, Alvin Wen Choong
Ma, Dong Rui
Lee, Seng Teik
Fong, Eileen
author_sort Tjin, Monica Suryana
title Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
title_short Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
title_full Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
title_fullStr Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
title_full_unstemmed Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
title_sort human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
publishDate 2014
url https://hdl.handle.net/10356/105284
http://hdl.handle.net/10220/20681
_version_ 1681056906288824320