Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats

Diabetes is one of the major chronic diseases diagnosed worldwide with a common complication of diabetic nephropathy (DN). There are multiple possible mechanisms associated with DN. Aldose reductase (AR) and Toll-like receptor 4 (TLR4) may be involved in the occurrence and development of DN. Here, w...

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Main Authors: Liu, Yang, Liu, Jing, Liu, Jianwei, Lin, Guimiao, Liu, Liwei, Liu, Xiaomin, Hu, Rui, Lian, Hongwei, Duan, Xiaojian, Yong, Ken-Tye, Ye, Ling
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/105953
http://hdl.handle.net/10220/26059
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1059532022-02-16T16:27:20Z Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats Liu, Yang Liu, Jing Liu, Jianwei Lin, Guimiao Liu, Liwei Liu, Xiaomin Hu, Rui Lian, Hongwei Duan, Xiaojian Yong, Ken-Tye Ye, Ling School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics Diabetes is one of the major chronic diseases diagnosed worldwide with a common complication of diabetic nephropathy (DN). There are multiple possible mechanisms associated with DN. Aldose reductase (AR) and Toll-like receptor 4 (TLR4) may be involved in the occurrence and development of DN. Here, we describe the distribution of AR and TLR4 in cells and renal tissues of diabetic rats through a quantum dot (QD)-based immunofluorescence technique and conventional immunohistochemistry. As a new type of nanosized fluorophore, QDs have been recognized in imaging applications and have broad prospects in biomedical research. The results of the reported study demonstrate that both the AR and the TLR4 proteins were upregulated in the renal tissues of diabetic rats. Further, to explore the relationship between AR and TLR4 in the pathogenesis of DN, a dual-color immunofluorescent labeling technique based on QDs was applied, where the expressions of AR and TLR4 in the renal tissues of diabetic rats were simultaneously observed – for the first time, as far as we are aware. The optimized QD-based immunofluorescence technique has not only shown a satisfying sensitivity and specificity for the detection of biomarkers in cells and tissues, but also is a valuable supplement of immunohistochemistry. The QD-based multiplexed imaging technology provides a new insight into the mechanistic study of the correlation among biological factors as well as having potential applications in the diagnosis and treatment of diseases. Published version 2015-06-25T01:46:22Z 2019-12-06T22:01:26Z 2015-06-25T01:46:22Z 2019-12-06T22:01:26Z 2015 2015 Journal Article Liu, X., Hu, R., Lian, H., Liu, Y., Liu, J., Liu, J., et al. Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats. International Journal of Nanomedicine, 10, 3651-3662. 1178-2013 https://hdl.handle.net/10356/105953 http://hdl.handle.net/10220/26059 10.2147/IJN.S81395 26056446 en International Journal of Nanomedicine © 2015 Liu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics
spellingShingle DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics
Liu, Yang
Liu, Jing
Liu, Jianwei
Lin, Guimiao
Liu, Liwei
Liu, Xiaomin
Hu, Rui
Lian, Hongwei
Duan, Xiaojian
Yong, Ken-Tye
Ye, Ling
Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
description Diabetes is one of the major chronic diseases diagnosed worldwide with a common complication of diabetic nephropathy (DN). There are multiple possible mechanisms associated with DN. Aldose reductase (AR) and Toll-like receptor 4 (TLR4) may be involved in the occurrence and development of DN. Here, we describe the distribution of AR and TLR4 in cells and renal tissues of diabetic rats through a quantum dot (QD)-based immunofluorescence technique and conventional immunohistochemistry. As a new type of nanosized fluorophore, QDs have been recognized in imaging applications and have broad prospects in biomedical research. The results of the reported study demonstrate that both the AR and the TLR4 proteins were upregulated in the renal tissues of diabetic rats. Further, to explore the relationship between AR and TLR4 in the pathogenesis of DN, a dual-color immunofluorescent labeling technique based on QDs was applied, where the expressions of AR and TLR4 in the renal tissues of diabetic rats were simultaneously observed – for the first time, as far as we are aware. The optimized QD-based immunofluorescence technique has not only shown a satisfying sensitivity and specificity for the detection of biomarkers in cells and tissues, but also is a valuable supplement of immunohistochemistry. The QD-based multiplexed imaging technology provides a new insight into the mechanistic study of the correlation among biological factors as well as having potential applications in the diagnosis and treatment of diseases.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Liu, Yang
Liu, Jing
Liu, Jianwei
Lin, Guimiao
Liu, Liwei
Liu, Xiaomin
Hu, Rui
Lian, Hongwei
Duan, Xiaojian
Yong, Ken-Tye
Ye, Ling
format Article
author Liu, Yang
Liu, Jing
Liu, Jianwei
Lin, Guimiao
Liu, Liwei
Liu, Xiaomin
Hu, Rui
Lian, Hongwei
Duan, Xiaojian
Yong, Ken-Tye
Ye, Ling
author_sort Liu, Yang
title Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
title_short Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
title_full Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
title_fullStr Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
title_full_unstemmed Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
title_sort dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and toll-like receptor 4 in the kidneys of diabetic rats
publishDate 2015
url https://hdl.handle.net/10356/105953
http://hdl.handle.net/10220/26059
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