Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation

Biofilms play an important role in the pathogenesis of Group A Streptococcus (GAS), a gram-positive pathogen responsible for a wide range infections and significant public health impact. Although most GAS serotypes are able to form biofilms, there is large heterogeneity between individual strains in...

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Main Authors: Matysik, Artur, Kline, Kimberly A.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/105966
http://hdl.handle.net/10220/48803
https://doi.org/10.21979/N9/P6T8F8
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1059662021-01-18T04:50:20Z Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation Matysik, Artur Kline, Kimberly A. School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering Streptococcus Pyogenes Biofilms DRNTU::Science::Biological sciences Biofilms play an important role in the pathogenesis of Group A Streptococcus (GAS), a gram-positive pathogen responsible for a wide range infections and significant public health impact. Although most GAS serotypes are able to form biofilms, there is large heterogeneity between individual strains in biofilm formation, as measured by standard crystal violet assays. It is generally accepted that biofilm formation includes initial adhesion of bacterial cells to a surface, followed by microcolony formation, biofilm maturation, and extensive production of extracellular matrix that links together proliferating cells and provides a scaffold for the three-dimensional biofilm structure. However, our studies show that for GAS strain JS95, microcolony formation is not an essential step in static biofilm formation, and instead, biofilm can be effectively formed from slow-growing or non-replicating late exponential or early stationary planktonic cells, via sedimentation and fixation of GAS chains into biofilms. In addition, we show that the GAS capsule specifically contributes to the alternative, sedimentation-initiated biofilms. Microcolony-independent, sedimentation biofilms are similar in morphology and 3-D structure to biofilms initiated by actively dividing planktonic bacteria. We conclude that GAS can form biofilms by an alternate, non-canonical mechanism that does not require transition from microcolony formation to biofilm maturation, and which may be obscured by biofilm phenotypes that arise via the classical biofilm maturation processes. NMRC (Natl Medical Research Council, S’pore) Accepted version 2019-06-18T07:52:45Z 2019-12-06T22:01:46Z 2019-06-18T07:52:45Z 2019-12-06T22:01:46Z 2019 Journal Article Matysik, A., & Kline, K. A. Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation. Journal of Bacteriology, JB.00052-19. doi:10.1128/JB.00052-19 0021-9193 https://hdl.handle.net/10356/105966 http://hdl.handle.net/10220/48803 10.1128/JB.00052-19 en Journal of Bacteriology https://doi.org/10.21979/N9/P6T8F8 © 2019 Matysik and Kline. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. 35 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Streptococcus Pyogenes
Biofilms
DRNTU::Science::Biological sciences
spellingShingle Streptococcus Pyogenes
Biofilms
DRNTU::Science::Biological sciences
Matysik, Artur
Kline, Kimberly A.
Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
description Biofilms play an important role in the pathogenesis of Group A Streptococcus (GAS), a gram-positive pathogen responsible for a wide range infections and significant public health impact. Although most GAS serotypes are able to form biofilms, there is large heterogeneity between individual strains in biofilm formation, as measured by standard crystal violet assays. It is generally accepted that biofilm formation includes initial adhesion of bacterial cells to a surface, followed by microcolony formation, biofilm maturation, and extensive production of extracellular matrix that links together proliferating cells and provides a scaffold for the three-dimensional biofilm structure. However, our studies show that for GAS strain JS95, microcolony formation is not an essential step in static biofilm formation, and instead, biofilm can be effectively formed from slow-growing or non-replicating late exponential or early stationary planktonic cells, via sedimentation and fixation of GAS chains into biofilms. In addition, we show that the GAS capsule specifically contributes to the alternative, sedimentation-initiated biofilms. Microcolony-independent, sedimentation biofilms are similar in morphology and 3-D structure to biofilms initiated by actively dividing planktonic bacteria. We conclude that GAS can form biofilms by an alternate, non-canonical mechanism that does not require transition from microcolony formation to biofilm maturation, and which may be obscured by biofilm phenotypes that arise via the classical biofilm maturation processes.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Matysik, Artur
Kline, Kimberly A.
format Article
author Matysik, Artur
Kline, Kimberly A.
author_sort Matysik, Artur
title Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
title_short Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
title_full Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
title_fullStr Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
title_full_unstemmed Streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
title_sort streptococcus pyogenes capsule promotes microcolony-independent biofilm formation
publishDate 2019
url https://hdl.handle.net/10356/105966
http://hdl.handle.net/10220/48803
https://doi.org/10.21979/N9/P6T8F8
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