The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation

Sludge fermentation between pH 4 and 11 was investigated to generate volatile fatty acids (VFA). Despite the highest sludge solubilization of 25.9% at pH 11, VFA accumulation was optimized at pH 8 (12.5% out of 13.1% sludge solubilization). 454 pyrosequencing identified wide diversity of acidogens i...

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Main Authors: Maspolim, Yogananda, Zhou, Yan, Guo, Chenghong, Xiao, Keke, Ng, Wun Jern
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/106226
http://hdl.handle.net/10220/26375
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1062262020-09-26T22:00:07Z The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern School of Civil and Environmental Engineering Public Utilities Board of Singapore Nanyang Environment and Water Research Institute DRNTU::Engineering::Environmental engineering::Waste management Sludge fermentation between pH 4 and 11 was investigated to generate volatile fatty acids (VFA). Despite the highest sludge solubilization of 25.9% at pH 11, VFA accumulation was optimized at pH 8 (12.5% out of 13.1% sludge solubilization). 454 pyrosequencing identified wide diversity of acidogens in bioreactors operated at the various pHs, with Tissierella, Petrimonas, Proteiniphilum, Levilinea, Proteiniborus and Sedimentibacter enriched and contributing to the enhanced fermentation at pH 8. Hydrolytic enzymatic assays determined abiotic effect to be the leading cause for improved solubilization under high alkaline condition but the environmental stress at pH 9 and above might lead to disrupt biological activities and eventually VFA production. Furthermore, molecular weight (MW) characterization of the soluble fractions found large MW aromatic substances at pH 9 and above, that is normally associated with poor biodegradability, making them disadvantageous for subsequent bioprocesses. The findings provided information to better understand and control sludge fermentation. NRF (Natl Research Foundation, S’pore) Accepted version 2015-07-10T08:30:12Z 2019-12-06T22:06:52Z 2015-07-10T08:30:12Z 2019-12-06T22:06:52Z 2015 2015 Journal Article Maspolim, Y., Zhou, Y., Guo, C., Xiao, K., & Ng, W. J. (2015). The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation. Bioresource technology, 190, 289-298. 0960-8524 https://hdl.handle.net/10356/106226 http://hdl.handle.net/10220/26375 10.1016/j.biortech.2015.04.087 en Bioresource technology © 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioresource Technology, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.biortech.2015.04.087]. 38 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Environmental engineering::Waste management
spellingShingle DRNTU::Engineering::Environmental engineering::Waste management
Maspolim, Yogananda
Zhou, Yan
Guo, Chenghong
Xiao, Keke
Ng, Wun Jern
The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
description Sludge fermentation between pH 4 and 11 was investigated to generate volatile fatty acids (VFA). Despite the highest sludge solubilization of 25.9% at pH 11, VFA accumulation was optimized at pH 8 (12.5% out of 13.1% sludge solubilization). 454 pyrosequencing identified wide diversity of acidogens in bioreactors operated at the various pHs, with Tissierella, Petrimonas, Proteiniphilum, Levilinea, Proteiniborus and Sedimentibacter enriched and contributing to the enhanced fermentation at pH 8. Hydrolytic enzymatic assays determined abiotic effect to be the leading cause for improved solubilization under high alkaline condition but the environmental stress at pH 9 and above might lead to disrupt biological activities and eventually VFA production. Furthermore, molecular weight (MW) characterization of the soluble fractions found large MW aromatic substances at pH 9 and above, that is normally associated with poor biodegradability, making them disadvantageous for subsequent bioprocesses. The findings provided information to better understand and control sludge fermentation.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Maspolim, Yogananda
Zhou, Yan
Guo, Chenghong
Xiao, Keke
Ng, Wun Jern
format Article
author Maspolim, Yogananda
Zhou, Yan
Guo, Chenghong
Xiao, Keke
Ng, Wun Jern
author_sort Maspolim, Yogananda
title The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
title_short The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
title_full The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
title_fullStr The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
title_full_unstemmed The effect of pH on solubilization of organic matter and microbial community structures in sludge fermentation
title_sort effect of ph on solubilization of organic matter and microbial community structures in sludge fermentation
publishDate 2015
url https://hdl.handle.net/10356/106226
http://hdl.handle.net/10220/26375
_version_ 1681057672215920640