Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics
Integrating a dissolvable membrane into a sensor allows the control of sample flow, location and duration in critical areas. These time-barrier films stop the flow of samples until the membrane has dissolved, thus, for example, allowing increased exposure time between immunoreagents for the formatio...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/106247 http://hdl.handle.net/10220/48914 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-106247 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1062472023-07-14T15:58:00Z Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics Yitian, Alicia Lu Eltzov, Evgeni Marks, Robert S. Tok, Alfred Iing Yoong Harpaz, Dorin Axelrod, Tim School of Materials Science & Engineering Institute for Sports Research DRNTU::Engineering::Materials Polyvinyl Alcohol Dissolvable Membrane Integrating a dissolvable membrane into a sensor allows the control of sample flow, location and duration in critical areas. These time-barrier films stop the flow of samples until the membrane has dissolved, thus, for example, allowing increased exposure time between immunoreagents for the formation of greater numbers of immuno-complexes, ensuring higher sensitivity, reactivity, and helping to reduce false-positive signals. In this study, dissolvable polyvinyl alcohol (PVA) films are used in a 3D-printed sensor holder, which enables film integration without the use of glue. PVA is a synthetic hydrophilic linear polymer, its solubility is dependent on its molecular weight and degree of hydrolysis. Three types of PVAs films were tested herein: (1) PVA 1-Mw: 30–70 K, 87–90% hydrolyzed; (2) PVA 2-Mw: 31–50 K, 98–99% hydrolyzed and (3) PVA 3-Mw: 89–98 K, >99% hydrolyzed. The films were exposed to water in (1) the novel 3D-printed holder and (2) directly immersed into a water droplet. After comparing the time taken to dissolve PVA 1–3 films, PVA 1 films of 5–20% (w/v) are found to be most suitable as time barrier films, due to their optimal dissolution times and physical properties for integration into the customized 3D-printed holder. NRF (Natl Research Foundation, S’pore) Published version 2019-06-21T08:46:48Z 2019-12-06T22:07:21Z 2019-06-21T08:46:48Z 2019-12-06T22:07:21Z 2019 Journal Article Harpaz, D., Axelrod, T., Yitian, A. L., Eltzov, E., Marks, R. S., & Tok, A. I. Y. (2019). Dissolvable Polyvinyl-Alcohol Film, a Time-Barrier to Modulate Sample Flow in a 3D-Printed Holder for Capillary Flow Paper Diagnostics. Materials, 12(3), 343-. doi:10.3390/ma12030343 1996-1944 https://hdl.handle.net/10356/106247 http://hdl.handle.net/10220/48914 10.3390/ma12030343 en Materials © 2019 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 11 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
DRNTU::Engineering::Materials Polyvinyl Alcohol Dissolvable Membrane |
spellingShingle |
DRNTU::Engineering::Materials Polyvinyl Alcohol Dissolvable Membrane Yitian, Alicia Lu Eltzov, Evgeni Marks, Robert S. Tok, Alfred Iing Yoong Harpaz, Dorin Axelrod, Tim Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
description |
Integrating a dissolvable membrane into a sensor allows the control of sample flow, location and duration in critical areas. These time-barrier films stop the flow of samples until the membrane has dissolved, thus, for example, allowing increased exposure time between immunoreagents for the formation of greater numbers of immuno-complexes, ensuring higher sensitivity, reactivity, and helping to reduce false-positive signals. In this study, dissolvable polyvinyl alcohol (PVA) films are used in a 3D-printed sensor holder, which enables film integration without the use of glue. PVA is a synthetic hydrophilic linear polymer, its solubility is dependent on its molecular weight and degree of hydrolysis. Three types of PVAs films were tested herein: (1) PVA 1-Mw: 30–70 K, 87–90% hydrolyzed; (2) PVA 2-Mw: 31–50 K, 98–99% hydrolyzed and (3) PVA 3-Mw: 89–98 K, >99% hydrolyzed. The films were exposed to water in (1) the novel 3D-printed holder and (2) directly immersed into a water droplet. After comparing the time taken to dissolve PVA 1–3 films, PVA 1 films of 5–20% (w/v) are found to be most suitable as time barrier films, due to their optimal dissolution times and physical properties for integration into the customized 3D-printed holder. |
author2 |
School of Materials Science & Engineering |
author_facet |
School of Materials Science & Engineering Yitian, Alicia Lu Eltzov, Evgeni Marks, Robert S. Tok, Alfred Iing Yoong Harpaz, Dorin Axelrod, Tim |
format |
Article |
author |
Yitian, Alicia Lu Eltzov, Evgeni Marks, Robert S. Tok, Alfred Iing Yoong Harpaz, Dorin Axelrod, Tim |
author_sort |
Yitian, Alicia Lu |
title |
Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
title_short |
Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
title_full |
Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
title_fullStr |
Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
title_full_unstemmed |
Dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
title_sort |
dissolvable polyvinyl-alcohol film, a time-barrier to modulate sample flow in a 3d-printed holder for capillary flow paper diagnostics |
publishDate |
2019 |
url |
https://hdl.handle.net/10356/106247 http://hdl.handle.net/10220/48914 |
_version_ |
1773551194188283904 |