Modeling pK shift in DNA triplexes containing locked nucleic acids

• Main Authors: Hartono, Yossa Dwi, Xu, You, Karshikoff, Andrey, Nilsson, Lennart, Villa, Alessandra
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: DNA Triplexes; Locked Nucleic Acids; Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/106398; http://hdl.handle.net/10220/49611
• Institution: Nanyang Technological University

The protonation states for nucleic acid bases are difficult to assess experimentally. In the context of DNA triplex, the protonation state of cytidine in the third strand is particularly important, because it needs to be protonated in order to form Hoogsteen hydrogen bonds. A sugar modification, loc...