Structure, sequence and ion-exchange of left-handed G-quadruplexes
Besides the canonical double-stranded helical B-DNA structure, DNA can form four-stranded G-quadruplex structures composed of stacked guanine G-tetrads. Until the recent first example of a left-handed structure named Z-G4, all solved G-quadruplex structures adopted a right-handed helical fold. We...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/106467 http://hdl.handle.net/10220/47993 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Besides the canonical double-stranded helical B-DNA structure, DNA can form four-stranded G-quadruplex structures composed of stacked guanine G-tetrads.
Until the recent first example of a left-handed structure named Z-G4, all solved G-quadruplex structures adopted a right-handed helical fold. We report on the discovery of five new left-handed G-quadruplex structures, including a minimal left-handed motif. These structures and associated findings help pave the way towards expanding the primary sequence requirements for left-handed G-quadruplexes.
Cations are important for G-quadruplex assembly as they bind between stacked G-tetrads. We have observed a cation coordinated in Z-G4 that has an exchange time on the order of one hour at room temperature. |
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