Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle

This paper proposes a remarkably facile staining protocol to visually investigate dynamic physiological events in insect tissues. We attempted to monitor Ca2+ dynamics during contraction of electrically stimulated living muscle. Advances in circuit miniaturization and insect neuromuscular physiology...

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Main Authors: Ferdinandus, Arai, Satoshi, Ishiwata, Shin’ichi, Suzuki, Madoka, Sato, Hirotaka
Other Authors: Lazzari, Claudio R.
Format: Article
Language:English
Published: 2015
Subjects:
Online Access:https://hdl.handle.net/10356/106633
http://hdl.handle.net/10220/25004
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1066332023-03-04T17:17:51Z Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle Ferdinandus Arai, Satoshi Ishiwata, Shin’ichi Suzuki, Madoka Sato, Hirotaka Lazzari, Claudio R. School of Mechanical and Aerospace Engineering DRNTU::Science::Biological sciences::Zoology::Invertebrates This paper proposes a remarkably facile staining protocol to visually investigate dynamic physiological events in insect tissues. We attempted to monitor Ca2+ dynamics during contraction of electrically stimulated living muscle. Advances in circuit miniaturization and insect neuromuscular physiology have enabled the hybridization of living insects and man-made electronic components, such as microcomputers, the result of which has been often referred as a Living Machine, Biohybrid, or Cyborg Insect. In order for Cyborg Insects to be of practical use, electrical stimulation parameters need to be optimized to induce desired muscle response (motor action) and minimize the damage in the muscle due to the electrical stimuli. Staining tissues and organs as well as measuring the dynamics of chemicals of interest in muscle should be conducted to quantitatively and systematically evaluate the effect of various stimulation parameters on the muscle response. However, existing staining processes require invasive surgery and/or arduous procedures using genetically encoded sensors. In this study, we developed a non-invasive and remarkably facile method for staining, in which chemical indicators can be orally administered (oral dosing). A chemical Ca2+ indicator was orally introduced into an insect of interest via food containing the chemical indicator and the indicator diffused from the insect digestion system to the target muscle tissue. We found that there was a positive relationship between the fluorescence intensity of the indicator and the frequency of electrical stimulation which indicates the orally dosed indicator successfully monitored Ca2+ dynamics in the muscle tissue. This oral dosing method has a potential to globally stain tissues including neurons, and investigating various physiological events in insects. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2015-02-02T06:08:50Z 2019-12-06T22:15:17Z 2015-02-02T06:08:50Z 2019-12-06T22:15:17Z 2015 2015 Journal Article Ferdinandus, Arai, S., Ishiwata, S., Suzuki, M., & Sato, H. (2015). Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle. PLOS One, 10(1). 1932-6203 https://hdl.handle.net/10356/106633 http://hdl.handle.net/10220/25004 10.1371/journal.pone.0116655 25590329 en PLOS One © 2015 Ferdinandus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Zoology::Invertebrates
spellingShingle DRNTU::Science::Biological sciences::Zoology::Invertebrates
Ferdinandus
Arai, Satoshi
Ishiwata, Shin’ichi
Suzuki, Madoka
Sato, Hirotaka
Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
description This paper proposes a remarkably facile staining protocol to visually investigate dynamic physiological events in insect tissues. We attempted to monitor Ca2+ dynamics during contraction of electrically stimulated living muscle. Advances in circuit miniaturization and insect neuromuscular physiology have enabled the hybridization of living insects and man-made electronic components, such as microcomputers, the result of which has been often referred as a Living Machine, Biohybrid, or Cyborg Insect. In order for Cyborg Insects to be of practical use, electrical stimulation parameters need to be optimized to induce desired muscle response (motor action) and minimize the damage in the muscle due to the electrical stimuli. Staining tissues and organs as well as measuring the dynamics of chemicals of interest in muscle should be conducted to quantitatively and systematically evaluate the effect of various stimulation parameters on the muscle response. However, existing staining processes require invasive surgery and/or arduous procedures using genetically encoded sensors. In this study, we developed a non-invasive and remarkably facile method for staining, in which chemical indicators can be orally administered (oral dosing). A chemical Ca2+ indicator was orally introduced into an insect of interest via food containing the chemical indicator and the indicator diffused from the insect digestion system to the target muscle tissue. We found that there was a positive relationship between the fluorescence intensity of the indicator and the frequency of electrical stimulation which indicates the orally dosed indicator successfully monitored Ca2+ dynamics in the muscle tissue. This oral dosing method has a potential to globally stain tissues including neurons, and investigating various physiological events in insects.
author2 Lazzari, Claudio R.
author_facet Lazzari, Claudio R.
Ferdinandus
Arai, Satoshi
Ishiwata, Shin’ichi
Suzuki, Madoka
Sato, Hirotaka
format Article
author Ferdinandus
Arai, Satoshi
Ishiwata, Shin’ichi
Suzuki, Madoka
Sato, Hirotaka
author_sort Ferdinandus
title Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
title_short Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
title_full Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
title_fullStr Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
title_full_unstemmed Oral dosing of chemical indicators for in vivo monitoring of Ca2+ dynamics in insect muscle
title_sort oral dosing of chemical indicators for in vivo monitoring of ca2+ dynamics in insect muscle
publishDate 2015
url https://hdl.handle.net/10356/106633
http://hdl.handle.net/10220/25004
_version_ 1759854972448538624