A novel non-sequencing approach for rapid authentication of Testudinis Carapax et Plastrum and Trionycis Carapax by species-specific primers

• Main Authors: Yang, Huan, Yu, Pingtian, Lu, Yi, Jiao, Zhaoqun, Chen, Liqun, Zhou, Ying, Shen, Yuping, Jia, Xiaobin
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: Polymerase Chain Reaction; Science::Biological sciences; Animal-derived Chinese Medicine
• Online Access: https://hdl.handle.net/10356/106669; http://hdl.handle.net/10220/49650
• Institution: Nanyang Technological University

A novel non-sequencing approach was developed to detect short DNA fragments (ca 100 bp) for rapid authentication of two natural products, namely Testudinis Carapax et Plastrum and Trionycis Carapax, based on the difference in mitochondrial genome. Five specifically designed primer reactions were est...