A polymeric cell stretching device for real-time imaging with optical microscopy

This paper reports the design, fabrication and characterization of a cell stretching device based on the side stretching approach. Numerical simulation using finite element method provides a guideline for optimizing the geometry and maximizing the output strain of the stretched membrane. An unique P...

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Main Authors: Huang, Yuli, Nguyen, Nam-Trung
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/106800
http://hdl.handle.net/10220/17599
http://dx.doi.org/10.1007/s10544-013-9796-2
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1068002019-12-06T22:18:38Z A polymeric cell stretching device for real-time imaging with optical microscopy Huang, Yuli Nguyen, Nam-Trung School of Mechanical and Aerospace Engineering This paper reports the design, fabrication and characterization of a cell stretching device based on the side stretching approach. Numerical simulation using finite element method provides a guideline for optimizing the geometry and maximizing the output strain of the stretched membrane. An unique PDMS-based micro fabrication process was developed for obtaining high parallelization, well controlled membrane thickness and an ultra-thin bottom layer that is crucial for the use with confocal microscopes. The stretching experiments are fully automated with both device actuation and image acquisition. A programmable pneumatic control system was built for simultaneous driving of 24 stretching arrays. The actuation signals are synchronized with the image acquisition system to obtain time-lapse recording of cells grown on the stretched membrane. Experimental results verified the characteristics predicted by the simulation. A platform with 15 stretching units was integrated on a standard 24 mm × 50 mm glass slide. Each unit can achieve a maximum strain of more than 60 %. The platform was tested for cell growth under cyclic stretching. The preliminary results show that the device is compatible with all standard microscopes. 2013-11-12T05:36:39Z 2019-12-06T22:18:38Z 2013-11-12T05:36:39Z 2019-12-06T22:18:38Z 2013 2013 Journal Article Huang, Y., & Nguyen, N. T. A polymeric cell stretching device for real-time imaging with optical microscopy. Biomedical Microdevices,in press. https://hdl.handle.net/10356/106800 http://hdl.handle.net/10220/17599 http://dx.doi.org/10.1007/s10544-013-9796-2 en Biomedical microdevices
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description This paper reports the design, fabrication and characterization of a cell stretching device based on the side stretching approach. Numerical simulation using finite element method provides a guideline for optimizing the geometry and maximizing the output strain of the stretched membrane. An unique PDMS-based micro fabrication process was developed for obtaining high parallelization, well controlled membrane thickness and an ultra-thin bottom layer that is crucial for the use with confocal microscopes. The stretching experiments are fully automated with both device actuation and image acquisition. A programmable pneumatic control system was built for simultaneous driving of 24 stretching arrays. The actuation signals are synchronized with the image acquisition system to obtain time-lapse recording of cells grown on the stretched membrane. Experimental results verified the characteristics predicted by the simulation. A platform with 15 stretching units was integrated on a standard 24 mm × 50 mm glass slide. Each unit can achieve a maximum strain of more than 60 %. The platform was tested for cell growth under cyclic stretching. The preliminary results show that the device is compatible with all standard microscopes.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Huang, Yuli
Nguyen, Nam-Trung
format Article
author Huang, Yuli
Nguyen, Nam-Trung
spellingShingle Huang, Yuli
Nguyen, Nam-Trung
A polymeric cell stretching device for real-time imaging with optical microscopy
author_sort Huang, Yuli
title A polymeric cell stretching device for real-time imaging with optical microscopy
title_short A polymeric cell stretching device for real-time imaging with optical microscopy
title_full A polymeric cell stretching device for real-time imaging with optical microscopy
title_fullStr A polymeric cell stretching device for real-time imaging with optical microscopy
title_full_unstemmed A polymeric cell stretching device for real-time imaging with optical microscopy
title_sort polymeric cell stretching device for real-time imaging with optical microscopy
publishDate 2013
url https://hdl.handle.net/10356/106800
http://hdl.handle.net/10220/17599
http://dx.doi.org/10.1007/s10544-013-9796-2
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