In-fiber photo-immobilization of a bioactive surface

• Main Authors: Lee, Elizabeth, Yong, Derrick, Yu, Xia, Li, Hao, Chan, Chi Chiu
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Medicine::Biomedical engineering
• Online Access: https://hdl.handle.net/10356/106937; http://hdl.handle.net/10220/25135
• Institution: Nanyang Technological University

We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.