Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexner...

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Main Authors: Liu, P. Y., Chin, Lip Ket, Ser, Wee, Ayi, T. C., Yap, P. H., Bourouina, T., Leprince-Wang, Y.
Other Authors: School of Electrical and Electronic Engineering
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Online Access:https://hdl.handle.net/10356/106996
http://hdl.handle.net/10220/25232
http://dx.doi.org/10.1016/j.proeng.2014.11.743
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1069962019-12-06T22:22:42Z Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry Liu, P. Y. Chin, Lip Ket Ser, Wee Ayi, T. C. Yap, P. H. Bourouina, T. Leprince-Wang, Y. School of Electrical and Electronic Engineering EUROSENSORS the European Conference on Solid-State Transducers (28th : 2014) DRNTU::Science::Biological sciences::Biophysics This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents. Published version 2015-03-11T06:36:23Z 2019-12-06T22:22:42Z 2015-03-11T06:36:23Z 2019-12-06T22:22:42Z 2014 2014 Conference Paper Liu, P. Y., Chin, L. K., Ser, W., Ayi, T. C., Yap, P. H., Bourouina, T., et al. (2014). Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry. Procedia engineering, 87, 356-359. https://hdl.handle.net/10356/106996 http://hdl.handle.net/10220/25232 http://dx.doi.org/10.1016/j.proeng.2014.11.743 en Procedia Engineering © 2014 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). 4 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Biophysics
spellingShingle DRNTU::Science::Biological sciences::Biophysics
Liu, P. Y.
Chin, Lip Ket
Ser, Wee
Ayi, T. C.
Yap, P. H.
Bourouina, T.
Leprince-Wang, Y.
Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
description This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Liu, P. Y.
Chin, Lip Ket
Ser, Wee
Ayi, T. C.
Yap, P. H.
Bourouina, T.
Leprince-Wang, Y.
format Conference or Workshop Item
author Liu, P. Y.
Chin, Lip Ket
Ser, Wee
Ayi, T. C.
Yap, P. H.
Bourouina, T.
Leprince-Wang, Y.
author_sort Liu, P. Y.
title Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
title_short Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
title_full Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
title_fullStr Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
title_full_unstemmed Real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
title_sort real-time measurement of single bacterium's refractive index using optofluidic immersion refractometry
publishDate 2015
url https://hdl.handle.net/10356/106996
http://hdl.handle.net/10220/25232
http://dx.doi.org/10.1016/j.proeng.2014.11.743
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