Understanding the biological functions of POPX2 phosphatase through its interactome
POPX2 (Partner of PIX 2) is a serine/threonine phosphatase known to dephosphorylate PAK1, CaMKII and TAK1. POPX2 has been reported to be positively associated with cell motility and invasiveness of breast cancer cells. In order to further investigate the roles and functions of POPX2 in the cells,...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
| Published: |
Nanyang Technological University
2020
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| Online Access: | https://hdl.handle.net/10356/136952 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | POPX2 (Partner of PIX 2) is a serine/threonine phosphatase known to dephosphorylate PAK1,
CaMKII and TAK1. POPX2 has been reported to be positively associated with cell motility and
invasiveness of breast cancer cells. In order to further investigate the roles and functions of
POPX2 in the cells, we have adopted two approaches to identify POPX2-interacting proteins. In
the first approach, we pulled down Flag-tagged POPX2 and determined the co-precipitated
proteins using mass spectrometry (MS). We discovered Coronin 1C (Coro 1C) as a potential
POPX2-interacting protein. In the second approach, we used a combination of SILAC-MS
proteomics and bioinformatic analysis and identified Check point kinase 1 (Chk1) as a binding
partner and possible substrate of POPX2. |
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