An effective recombinant protein expression and purification system in Saccharomyces cerevisiae

• Main Authors: Xie, Ying, Han, Xiao, Miao, Yansong
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Biological sciences; Actin Filament; Budding Yeast
• Online Access: https://hdl.handle.net/10356/137139
• Institution: Nanyang Technological University

The expression and purification of recombinant proteins using bacterial vectors is a mature and preferred system to obtain folded and stable proteins. However, functional post‐translational protein modifications, such as glycosylation or phosphorylation, can only be achieved using eukaryotic express...