Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window ha...
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sg-ntu-dr.10356-1371502023-12-29T06:50:31Z Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window Yin, Chao Wen, Guohua Liu, Chao Yang, Boguang Lin, Sien Huang, Jiawei Zhao, Pengchao Wong, Dexter Siu Hong Zhang, Kunyu Chen, Xiaoyu Li, Gang Jiang, Xiaohua Huang, Jianping Pu, Kanyi Wang, Lidai Bian, Liming School of Chemical and Biomedical Engineering Engineering::Chemical engineering Organic Semiconducting Polymer Nanoparticle Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies. MOE (Min. of Education, S’pore) Accepted version 2020-03-03T03:18:57Z 2020-03-03T03:18:57Z 2018 Journal Article Yin, C., Wen, G., Liu, C., Yang, B., Lin, S., Huang, J., . . . Bian, L. (2018). Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window. ACS Nano, 12(12), 12201-12211. doi:10.1021/acsnano.8b05906 1936-0851 https://hdl.handle.net/10356/137150 10.1021/acsnano.8b05906 30433761 2-s2.0-85057559129 12 12 12201 12211 en ACS nano This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.8b05906 application/pdf |
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Engineering::Chemical engineering Organic Semiconducting Polymer Nanoparticle Yin, Chao Wen, Guohua Liu, Chao Yang, Boguang Lin, Sien Huang, Jiawei Zhao, Pengchao Wong, Dexter Siu Hong Zhang, Kunyu Chen, Xiaoyu Li, Gang Jiang, Xiaohua Huang, Jianping Pu, Kanyi Wang, Lidai Bian, Liming Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
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Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Yin, Chao Wen, Guohua Liu, Chao Yang, Boguang Lin, Sien Huang, Jiawei Zhao, Pengchao Wong, Dexter Siu Hong Zhang, Kunyu Chen, Xiaoyu Li, Gang Jiang, Xiaohua Huang, Jianping Pu, Kanyi Wang, Lidai Bian, Liming |
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Article |
author |
Yin, Chao Wen, Guohua Liu, Chao Yang, Boguang Lin, Sien Huang, Jiawei Zhao, Pengchao Wong, Dexter Siu Hong Zhang, Kunyu Chen, Xiaoyu Li, Gang Jiang, Xiaohua Huang, Jianping Pu, Kanyi Wang, Lidai Bian, Liming |
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Yin, Chao |
title |
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
title_short |
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
title_full |
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
title_fullStr |
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
title_full_unstemmed |
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
title_sort |
organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window |
publishDate |
2020 |
url |
https://hdl.handle.net/10356/137150 |
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1787136675472736256 |