Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window

Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window ha...

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Main Authors: Yin, Chao, Wen, Guohua, Liu, Chao, Yang, Boguang, Lin, Sien, Huang, Jiawei, Zhao, Pengchao, Wong, Dexter Siu Hong, Zhang, Kunyu, Chen, Xiaoyu, Li, Gang, Jiang, Xiaohua, Huang, Jianping, Pu, Kanyi, Wang, Lidai, Bian, Liming
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/137150
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1371502023-12-29T06:50:31Z Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window Yin, Chao Wen, Guohua Liu, Chao Yang, Boguang Lin, Sien Huang, Jiawei Zhao, Pengchao Wong, Dexter Siu Hong Zhang, Kunyu Chen, Xiaoyu Li, Gang Jiang, Xiaohua Huang, Jianping Pu, Kanyi Wang, Lidai Bian, Liming School of Chemical and Biomedical Engineering Engineering::Chemical engineering Organic Semiconducting Polymer Nanoparticle Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies. MOE (Min. of Education, S’pore) Accepted version 2020-03-03T03:18:57Z 2020-03-03T03:18:57Z 2018 Journal Article Yin, C., Wen, G., Liu, C., Yang, B., Lin, S., Huang, J., . . . Bian, L. (2018). Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window. ACS Nano, 12(12), 12201-12211. doi:10.1021/acsnano.8b05906 1936-0851 https://hdl.handle.net/10356/137150 10.1021/acsnano.8b05906 30433761 2-s2.0-85057559129 12 12 12201 12211 en ACS nano This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.8b05906 application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
Organic Semiconducting Polymer
Nanoparticle
spellingShingle Engineering::Chemical engineering
Organic Semiconducting Polymer
Nanoparticle
Yin, Chao
Wen, Guohua
Liu, Chao
Yang, Boguang
Lin, Sien
Huang, Jiawei
Zhao, Pengchao
Wong, Dexter Siu Hong
Zhang, Kunyu
Chen, Xiaoyu
Li, Gang
Jiang, Xiaohua
Huang, Jianping
Pu, Kanyi
Wang, Lidai
Bian, Liming
Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
description Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Yin, Chao
Wen, Guohua
Liu, Chao
Yang, Boguang
Lin, Sien
Huang, Jiawei
Zhao, Pengchao
Wong, Dexter Siu Hong
Zhang, Kunyu
Chen, Xiaoyu
Li, Gang
Jiang, Xiaohua
Huang, Jianping
Pu, Kanyi
Wang, Lidai
Bian, Liming
format Article
author Yin, Chao
Wen, Guohua
Liu, Chao
Yang, Boguang
Lin, Sien
Huang, Jiawei
Zhao, Pengchao
Wong, Dexter Siu Hong
Zhang, Kunyu
Chen, Xiaoyu
Li, Gang
Jiang, Xiaohua
Huang, Jianping
Pu, Kanyi
Wang, Lidai
Bian, Liming
author_sort Yin, Chao
title Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
title_short Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
title_full Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
title_fullStr Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
title_full_unstemmed Organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
title_sort organic semiconducting polymer nanoparticles for photoacoustic labeling and tracking of stem cells in the second near-infrared window
publishDate 2020
url https://hdl.handle.net/10356/137150
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