Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”

Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible cont...

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Main Authors: Cai, Pingqiang, Li, Zhuyun, Keneth, Ela Sachyani, Wang, Luying, Wan, Changjin, Jiang, Ying, Hu, Benhui, Wu, Yun-Long, Wang, Shutao, Lim, Chwee Teck, Makeyev, Eugene V., Magdassi, Shlomo, Chen, Xiaodong
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/137874
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1378742023-07-14T15:57:22Z Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” Cai, Pingqiang Li, Zhuyun Keneth, Ela Sachyani Wang, Luying Wan, Changjin Jiang, Ying Hu, Benhui Wu, Yun-Long Wang, Shutao Lim, Chwee Teck Makeyev, Eugene V. Magdassi, Shlomo Chen, Xiaodong School of Materials Science & Engineering Innovative Center for Flexible Devices (iFLEX) Science::Biological sciences 3D Printing Epithelial Homeostasis Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer. NRF (Natl Research Foundation, S’pore) Accepted version 2020-04-17T02:21:47Z 2020-04-17T02:21:47Z 2019 Journal Article Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514 0935-9648 https://hdl.handle.net/10356/137874 10.1002/adma.201900514 31081206 2-s2.0-85065706324 28 31 en Advanced materials This is the peer reviewed version of the following article: Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514, which has been published in final form at https://doi.org/10.1002/adma.201900514. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
3D Printing
Epithelial Homeostasis
spellingShingle Science::Biological sciences
3D Printing
Epithelial Homeostasis
Cai, Pingqiang
Li, Zhuyun
Keneth, Ela Sachyani
Wang, Luying
Wan, Changjin
Jiang, Ying
Hu, Benhui
Wu, Yun-Long
Wang, Shutao
Lim, Chwee Teck
Makeyev, Eugene V.
Magdassi, Shlomo
Chen, Xiaodong
Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
description Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Cai, Pingqiang
Li, Zhuyun
Keneth, Ela Sachyani
Wang, Luying
Wan, Changjin
Jiang, Ying
Hu, Benhui
Wu, Yun-Long
Wang, Shutao
Lim, Chwee Teck
Makeyev, Eugene V.
Magdassi, Shlomo
Chen, Xiaodong
format Article
author Cai, Pingqiang
Li, Zhuyun
Keneth, Ela Sachyani
Wang, Luying
Wan, Changjin
Jiang, Ying
Hu, Benhui
Wu, Yun-Long
Wang, Shutao
Lim, Chwee Teck
Makeyev, Eugene V.
Magdassi, Shlomo
Chen, Xiaodong
author_sort Cai, Pingqiang
title Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
title_short Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
title_full Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
title_fullStr Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
title_full_unstemmed Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
title_sort differential homeostasis of sessile and pendant epithelium reconstituted in a 3d‐printed “geminichip”
publishDate 2020
url https://hdl.handle.net/10356/137874
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