Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique

Over the past decades, many researches have been done to design a delivery platform for nitric oxide (NO) that can release NO in a steady and long-lasting manner for the purpose of biomedical therapies such as promoting angiogenesis for wound healing. One proposed design is by using diatomaceous...

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Main Author: Yendri, Sheilla
Other Authors: Dalton Tay Chor Yong
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2020
Subjects:
Online Access:https://hdl.handle.net/10356/138866
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1388662023-03-04T15:47:30Z Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique Yendri, Sheilla Dalton Tay Chor Yong School of Materials Science and Engineering cytay@ntu.edu.sg Engineering::Materials::Biomaterials Over the past decades, many researches have been done to design a delivery platform for nitric oxide (NO) that can release NO in a steady and long-lasting manner for the purpose of biomedical therapies such as promoting angiogenesis for wound healing. One proposed design is by using diatomaceous earth (DE) porous surface modification to accommodate the NO donors. However, additional adjustments are needed to optimize the usage of DE as an ideal NO delivery platform. In this report, the executed adjustments were varying the thiol group concentration and applying two coating methods in order to achieve the best NO loaded DE delivery vehicle performance in terms of NO loading capacity and NO release profile. The two coating methods used were alginate encapsulation and layer-by-layer (LBL) technique. Compared to the encapsulation method, layer-by-layer technique is shown to provide a much steadier NO release profile that can last for up to 2 weeks. In addition, the exposure of LBL coating also reduces the cytotoxicity of NO loaded DE for 24 hours. The performance results of these two methods were quantified by plotting their NO cumulative release profile and testing their cell viability. Bachelor of Engineering (Materials Engineering) 2020-05-13T07:19:26Z 2020-05-13T07:19:26Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/138866 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials::Biomaterials
spellingShingle Engineering::Materials::Biomaterials
Yendri, Sheilla
Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
description Over the past decades, many researches have been done to design a delivery platform for nitric oxide (NO) that can release NO in a steady and long-lasting manner for the purpose of biomedical therapies such as promoting angiogenesis for wound healing. One proposed design is by using diatomaceous earth (DE) porous surface modification to accommodate the NO donors. However, additional adjustments are needed to optimize the usage of DE as an ideal NO delivery platform. In this report, the executed adjustments were varying the thiol group concentration and applying two coating methods in order to achieve the best NO loaded DE delivery vehicle performance in terms of NO loading capacity and NO release profile. The two coating methods used were alginate encapsulation and layer-by-layer (LBL) technique. Compared to the encapsulation method, layer-by-layer technique is shown to provide a much steadier NO release profile that can last for up to 2 weeks. In addition, the exposure of LBL coating also reduces the cytotoxicity of NO loaded DE for 24 hours. The performance results of these two methods were quantified by plotting their NO cumulative release profile and testing their cell viability.
author2 Dalton Tay Chor Yong
author_facet Dalton Tay Chor Yong
Yendri, Sheilla
format Final Year Project
author Yendri, Sheilla
author_sort Yendri, Sheilla
title Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
title_short Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
title_full Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
title_fullStr Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
title_full_unstemmed Optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
title_sort optimizing usage of diatomaceous earth as nitric oxide delivery platform : introduction of layer-by-layer coating technique
publisher Nanyang Technological University
publishDate 2020
url https://hdl.handle.net/10356/138866
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