Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy

• Main Authors: Hsieh, Chao-Mao, Liu, Patricia Yang, Chin, Lip Ket, Zhang, Jing Bo, Wang, Kuan, Sung, Kung-Bin, Ser, Wee, Bourouina, Tarik, Leprince-Wang, Yamin, Liu, Ai-Qun
• Other Authors: School of Electrical and Electronic Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Engineering::Electrical and electronic engineering; Optofluidics; Raman
• Online Access: https://hdl.handle.net/10356/138873
• Institution: Nanyang Technological University

Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.