Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro
Engineered nanoparticles (NPs) such as TiO2 and ZnO are key UV-blocking ingredients in sunscreens. While toxicological risks of applying these materials are generally regarded as low due to minute levels of penetration across the skin, our understanding of the physiological influence of potential ce...
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sg-ntu-dr.10356-1393662023-07-14T16:04:21Z Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro Gautam, Archana Rakshit, Moumita Nguyen, Kim Truc Kathawala, Mustafa Hussain Nguyen, Luong Thi Hien Tay, Chor Yong Wong, Esther Ng, Kee Woei School of Materials Science & Engineering School of Biological Sciences Environmental Chemistry and Materials Centre Nanyang Environment and Water Research Institute Science::Biological sciences Keratinocytes Sunscreen Engineered nanoparticles (NPs) such as TiO2 and ZnO are key UV-blocking ingredients in sunscreens. While toxicological risks of applying these materials are generally regarded as low due to minute levels of penetration across the skin, our understanding of the physiological influence of potential cell-nanoparticle interactions in the skin is limited. This study plugs the current knowledge gap by profiling TiO2 and ZnO NP interaction with primary human epidermal keratinocytes, based on potential levels of NP penetration across the skin. Specific attention was given to profiling real-life relevant levels of exposure, and accurate dosimetry measurements in vitro. ZnO was expectedly more cytotoxic than TiO2. Although both NPs generated Reactive Oxygen Species (ROS) and Mitochondrial Superoxide (MSO) within 4 h exposure to sub-lethal concentrations, ZnO induction of these oxidative stress markers increased much more significantly after 24 h exposure. Exposure to increasing NP concentrations increased autophagy induction along with activation of inflammatory responses in the keratinocytes, primarily through the TRAF6-mediated pathway. Sustained induction of autophagy led to degradation of TRAF6 and, only in the case of TiO2, reduced NF-κB activation. This pro-survival mode of autophagy induction provides further insights into the on-going debate on the use of these NPs in consumer products. Accepted version 2020-05-19T05:18:51Z 2020-05-19T05:18:51Z 2019 Journal Article Gautam, A., Rakshit, M., Nguyen, K. T., Kathawala, M. H., Nguyen, L. T. H., Tay, C. Y., . . ., Ng, K. W. (2019). Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro. NanoImpact, 15, 100177-. doi:10.1016/j.impact.2019.100177 2452-0748 https://hdl.handle.net/10356/139366 10.1016/j.impact.2019.100177 2-s2.0-85068852421 15 en NanoImpact © 2019 Elsevier B.V. All rights reserved. This paper was published in NanoImpact and is made available with permission of Elsevier B.V. application/pdf |
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Science::Biological sciences Keratinocytes Sunscreen Gautam, Archana Rakshit, Moumita Nguyen, Kim Truc Kathawala, Mustafa Hussain Nguyen, Luong Thi Hien Tay, Chor Yong Wong, Esther Ng, Kee Woei Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
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Engineered nanoparticles (NPs) such as TiO2 and ZnO are key UV-blocking ingredients in sunscreens. While toxicological risks of applying these materials are generally regarded as low due to minute levels of penetration across the skin, our understanding of the physiological influence of potential cell-nanoparticle interactions in the skin is limited. This study plugs the current knowledge gap by profiling TiO2 and ZnO NP interaction with primary human epidermal keratinocytes, based on potential levels of NP penetration across the skin. Specific attention was given to profiling real-life relevant levels of exposure, and accurate dosimetry measurements in vitro. ZnO was expectedly more cytotoxic than TiO2. Although both NPs generated Reactive Oxygen Species (ROS) and Mitochondrial Superoxide (MSO) within 4 h exposure to sub-lethal concentrations, ZnO induction of these oxidative stress markers increased much more significantly after 24 h exposure. Exposure to increasing NP concentrations increased autophagy induction along with activation of inflammatory responses in the keratinocytes, primarily through the TRAF6-mediated pathway. Sustained induction of autophagy led to degradation of TRAF6 and, only in the case of TiO2, reduced NF-κB activation. This pro-survival mode of autophagy induction provides further insights into the on-going debate on the use of these NPs in consumer products. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Gautam, Archana Rakshit, Moumita Nguyen, Kim Truc Kathawala, Mustafa Hussain Nguyen, Luong Thi Hien Tay, Chor Yong Wong, Esther Ng, Kee Woei |
format |
Article |
author |
Gautam, Archana Rakshit, Moumita Nguyen, Kim Truc Kathawala, Mustafa Hussain Nguyen, Luong Thi Hien Tay, Chor Yong Wong, Esther Ng, Kee Woei |
author_sort |
Gautam, Archana |
title |
Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
title_short |
Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
title_full |
Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
title_fullStr |
Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
title_full_unstemmed |
Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
title_sort |
understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro |
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2020 |
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https://hdl.handle.net/10356/139366 |
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1773551396565549056 |