Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures
Ever since its development, tissue engineering has played a significant role in the medical arena with an ever-growing demand for various tissue donations. One crucial factor in conducting in vitro tissue engineering study is the construction of a desirable artificial three-dimensional (3D) hydrogel...
Saved in:
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/139433 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-139433 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1394332020-05-19T07:59:56Z Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures Hu, Kan An, Jianing Yoon, Yong-Jin School of Mechanical and Aerospace Engineering Singapore-MIT Alliance Programme Engineering::Mechanical engineering Hydrogel Microstructures Ever since its development, tissue engineering has played a significant role in the medical arena with an ever-growing demand for various tissue donations. One crucial factor in conducting in vitro tissue engineering study is the construction of a desirable artificial three-dimensional (3D) hydrogel tissue scaffold to act as the extracellular matrix (ECM), meeting the complex requirements for specific cell cultures. Existing hydrogel scaffold fabrication techniques and systems utilized in constructing ECM are either twodimensionally limiting, hard to control the pattern morphologies or expensive and time consuming. In the present study, we introduce a simple, inexpensive method for selective patterning 3D porous microstructures. This technique-'two wavelength photo-initiation and photo inhibition competes’ is an extension of conventional photo-patterning method. Integrating with shadow mask, photo inhibition radicals were introduced to couple with the polymerization chains and terminate the photo crosslinking behavior at designed region, making 3D selectively patterning hydrogel feasible. High aspect ratio ridge with selectively inhibited porous structures and selectively patterned micro pillar were fabricated using this method within 1 minute. The in vitro cell test results indicate the patterned structures' good biocompatibility. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) 2020-05-19T07:59:56Z 2020-05-19T07:59:56Z 2018 Journal Article Hu, K., An, J., & Yoon, Y.-J. (2018). Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures. International Journal of Precision Engineering and Manufacturing, 19(5), 729-735. doi:10.1007/s12541-018-0087-y 2234-7593 https://hdl.handle.net/10356/139433 10.1007/s12541-018-0087-y 2-s2.0-85047221530 5 19 729 735 en International Journal of Precision Engineering and Manufacturing © 2018 KSPE and Springer (Published by Springer). All rights reserved. |
institution |
Nanyang Technological University |
building |
NTU Library |
country |
Singapore |
collection |
DR-NTU |
language |
English |
topic |
Engineering::Mechanical engineering Hydrogel Microstructures |
spellingShingle |
Engineering::Mechanical engineering Hydrogel Microstructures Hu, Kan An, Jianing Yoon, Yong-Jin Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
description |
Ever since its development, tissue engineering has played a significant role in the medical arena with an ever-growing demand for various tissue donations. One crucial factor in conducting in vitro tissue engineering study is the construction of a desirable artificial three-dimensional (3D) hydrogel tissue scaffold to act as the extracellular matrix (ECM), meeting the complex requirements for specific cell cultures. Existing hydrogel scaffold fabrication techniques and systems utilized in constructing ECM are either twodimensionally limiting, hard to control the pattern morphologies or expensive and time consuming. In the present study, we introduce a simple, inexpensive method for selective patterning 3D porous microstructures. This technique-'two wavelength photo-initiation and photo inhibition competes’ is an extension of conventional photo-patterning method. Integrating with shadow mask, photo inhibition radicals were introduced to couple with the polymerization chains and terminate the photo crosslinking behavior at designed region, making 3D selectively patterning hydrogel feasible. High aspect ratio ridge with selectively inhibited porous structures and selectively patterned micro pillar were fabricated using this method within 1 minute. The in vitro cell test results indicate the patterned structures' good biocompatibility. |
author2 |
School of Mechanical and Aerospace Engineering |
author_facet |
School of Mechanical and Aerospace Engineering Hu, Kan An, Jianing Yoon, Yong-Jin |
format |
Article |
author |
Hu, Kan An, Jianing Yoon, Yong-Jin |
author_sort |
Hu, Kan |
title |
Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
title_short |
Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
title_full |
Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
title_fullStr |
Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
title_full_unstemmed |
Two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
title_sort |
two-wavelength, photo-initiation and photo-inhibition competing for selective photo-patterning of hydrogel porous microstructures |
publishDate |
2020 |
url |
https://hdl.handle.net/10356/139433 |
_version_ |
1681058226504728576 |