Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment

Matrix nanotopography plays an important role in regulating cell behaviors by providing spatial as well as mechanical cues for cells to sense. It has been proposed that nanoscale topography is possible to modulate the tensions which direct the formation of cytoskeleton and the organization of the me...

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Main Authors: Han, Jingjing, Lin, Keng-Hui, Chew, Lock Yue
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2020
Subjects:
3D
Online Access:https://hdl.handle.net/10356/139712
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1397122020-05-21T04:22:59Z Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment Han, Jingjing Lin, Keng-Hui Chew, Lock Yue School of Physical and Mathematical Sciences Complexity Institute Science::Physics 3D Surface Curvature Matrix nanotopography plays an important role in regulating cell behaviors by providing spatial as well as mechanical cues for cells to sense. It has been proposed that nanoscale topography is possible to modulate the tensions which direct the formation of cytoskeleton and the organization of the membrane receptor within the cell, which in turn regulate intracellular mechanical and biochemical signaling. With current studies on this topic being performed mainly in 2D platforms, the question on how nanotopography can influence cell bahaviors in 3D environments has yet to be addressed. In this paper, we explored this question by placing cells in 3D hollow spherical polydimethylsiloxane scaffolds. After culturing rat embryonic fibroblast cells in two kinds of scaffold, one with smooth surface and the other with numerous nano-spikes, we observed that cells in the smooth scaffold have more anchoring sites and more focal adhesions than in the etched scaffold. Moreover, we found the presence of correlation between cortical actin, the important component for supporting cell attachment, and local cell geometry. MOE (Min. of Education, S’pore) 2020-05-21T04:22:58Z 2020-05-21T04:22:58Z 2017 Journal Article Han, J., Lin, K.-H., & Chew, L. Y. (2017). Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment. Journal of Physics: Condensed Matter, 29(45), 455101-. doi:10.1088/1361-648x/aa8d49 0953-8984 https://hdl.handle.net/10356/139712 10.1088/1361-648X/aa8d49 28920917 2-s2.0-85038890158 45 29 en Journal of Physics: Condensed Matter © 2017 IOP Publishing Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Science::Physics
3D
Surface Curvature
spellingShingle Science::Physics
3D
Surface Curvature
Han, Jingjing
Lin, Keng-Hui
Chew, Lock Yue
Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
description Matrix nanotopography plays an important role in regulating cell behaviors by providing spatial as well as mechanical cues for cells to sense. It has been proposed that nanoscale topography is possible to modulate the tensions which direct the formation of cytoskeleton and the organization of the membrane receptor within the cell, which in turn regulate intracellular mechanical and biochemical signaling. With current studies on this topic being performed mainly in 2D platforms, the question on how nanotopography can influence cell bahaviors in 3D environments has yet to be addressed. In this paper, we explored this question by placing cells in 3D hollow spherical polydimethylsiloxane scaffolds. After culturing rat embryonic fibroblast cells in two kinds of scaffold, one with smooth surface and the other with numerous nano-spikes, we observed that cells in the smooth scaffold have more anchoring sites and more focal adhesions than in the etched scaffold. Moreover, we found the presence of correlation between cortical actin, the important component for supporting cell attachment, and local cell geometry.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Han, Jingjing
Lin, Keng-Hui
Chew, Lock Yue
format Article
author Han, Jingjing
Lin, Keng-Hui
Chew, Lock Yue
author_sort Han, Jingjing
title Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
title_short Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
title_full Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
title_fullStr Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
title_full_unstemmed Study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3D environment
title_sort study on the regulation of focal adesions and cortical actin by matrix nanotopography in 3d environment
publishDate 2020
url https://hdl.handle.net/10356/139712
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