Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells

Enzymes are promising electrocatalysts for electron transfer (ET) in many biological processes. Strategies to enhance ET between enzymes and electroactive surfaces include orientation and immobilization of the enzymes and electron mediation. Here, we develop a strategy to couple orientation and elec...

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Main Authors: Elouarzaki, Kamal, Cheng, Daojian, Fisher, Adrian C., Lee, Jong-Min
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/140492
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1404922020-05-29T08:32:01Z Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells Elouarzaki, Kamal Cheng, Daojian Fisher, Adrian C. Lee, Jong-Min School of Chemical and Biomedical Engineering Engineering::Chemical engineering Chemical Engineering Electrochemistry Enzymes are promising electrocatalysts for electron transfer (ET) in many biological processes. Strategies to enhance ET between enzymes and electroactive surfaces include orientation and immobilization of the enzymes and electron mediation. Here, we develop a strategy to couple orientation and electron mediation on electrodes based on carbon nanotubes. This is achieved by the synthesis of a redox mediator that contains an enzyme-orientation site (pyrene), an electron-carrier redox mediator (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)) and an electropolymerizable monomer (pyrrole). The coupling of an enzymatic orientation and a mediated ET in the same chemical structure (pyrrole–ABTS–pyrene (pyrr–ABTS–pyr)) provides a much-improved performance in the bioelectrocatalysis. We demonstrate two fuel cells for the synthesized redox mediator. In a proton-exchange membrane hydrogen/air fuel cell and in a membraneless fuel cell, the pyrr–ABTS–pyr biocathode provides a power density of 1.07 mW cm−2 and 7.9 mW cm−2, respectively. The principle of coupling an enzyme orientation and a redox mediator allows a great variety of mediators to be engineered and provides vast possibilities for the development of fuel cells. NRF (Natl Research Foundation, S’pore) 2020-05-29T08:32:01Z 2020-05-29T08:32:01Z 2018 Journal Article Elouarzaki, K., Cheng, D., Fishers, A. C., & Lee, J.-M. (2018). Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells. Nature Energy, 3(7), 574-581. doi:10.1038/s41560-018-0166-4 2058-7546 https://hdl.handle.net/10356/140492 10.1038/s41560-018-0166-4 2-s2.0-85047972378 7 3 574 581 en Nature Energy © 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Engineering::Chemical engineering
Chemical Engineering
Electrochemistry
spellingShingle Engineering::Chemical engineering
Chemical Engineering
Electrochemistry
Elouarzaki, Kamal
Cheng, Daojian
Fisher, Adrian C.
Lee, Jong-Min
Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
description Enzymes are promising electrocatalysts for electron transfer (ET) in many biological processes. Strategies to enhance ET between enzymes and electroactive surfaces include orientation and immobilization of the enzymes and electron mediation. Here, we develop a strategy to couple orientation and electron mediation on electrodes based on carbon nanotubes. This is achieved by the synthesis of a redox mediator that contains an enzyme-orientation site (pyrene), an electron-carrier redox mediator (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)) and an electropolymerizable monomer (pyrrole). The coupling of an enzymatic orientation and a mediated ET in the same chemical structure (pyrrole–ABTS–pyrene (pyrr–ABTS–pyr)) provides a much-improved performance in the bioelectrocatalysis. We demonstrate two fuel cells for the synthesized redox mediator. In a proton-exchange membrane hydrogen/air fuel cell and in a membraneless fuel cell, the pyrr–ABTS–pyr biocathode provides a power density of 1.07 mW cm−2 and 7.9 mW cm−2, respectively. The principle of coupling an enzyme orientation and a redox mediator allows a great variety of mediators to be engineered and provides vast possibilities for the development of fuel cells.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Elouarzaki, Kamal
Cheng, Daojian
Fisher, Adrian C.
Lee, Jong-Min
format Article
author Elouarzaki, Kamal
Cheng, Daojian
Fisher, Adrian C.
Lee, Jong-Min
author_sort Elouarzaki, Kamal
title Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
title_short Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
title_full Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
title_fullStr Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
title_full_unstemmed Coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
title_sort coupling orientation and mediation strategies for efficient electron transfer in hybrid biofuel cells
publishDate 2020
url https://hdl.handle.net/10356/140492
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