Engineering biofilms for efficient removal of arsenic from water

One of the most fundamental necessity for mankind – clean water, is gradually decreasing as the world population increases beyond 7 billion. Arsenic contaminated groundwater is one of the main concerns, given its carcinogenic nature and widespread in waterbodies across the world. Various methods has...

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Main Author: Chen, Celestine Yi Ru
Other Authors: Cao Bin
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2020
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Online Access:https://hdl.handle.net/10356/140519
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1405192020-05-29T11:40:13Z Engineering biofilms for efficient removal of arsenic from water Chen, Celestine Yi Ru Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences and Engineering bincao@ntu.edu.sg Engineering::Environmental engineering One of the most fundamental necessity for mankind – clean water, is gradually decreasing as the world population increases beyond 7 billion. Arsenic contaminated groundwater is one of the main concerns, given its carcinogenic nature and widespread in waterbodies across the world. Various methods has been developed for arsenic removal, however, the application of these technologies is more often than not beyond the capacity of developing nations. In light of this, a research on the Shewanella oneidensis MR-1 strain was conducted to explore the feasibility of bacterial sorption of groundwater arsenic for the cleansing of water. S. oneidensis is a bacterium known for its functional protein, BpfA, which promotes the growth of biofilm. The innate ability of S. oneidensis to form biofilm for the sorption of arsenic is a more affordable method, however there is a threshold concentration limit to the extent of sorption. Hence, we explore the viability of modifying the genetics of the bacterium to enhance its sorption capacity. A mutant, BK3, was genetically-modified to express functional peptide for arsenic sorption on cell surface and was experimented in comparison with the wild type to differentiate their biofilm formation performance. In this report, the performance of wild type and BK3 in their biofilm and planktonic states will be discussed to determine the optimal conditions for sorption, and thus removal of arsenic. Bachelor of Engineering (Environmental Engineering) 2020-05-29T11:40:13Z 2020-05-29T11:40:13Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/140519 en EN-03 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Engineering::Environmental engineering
spellingShingle Engineering::Environmental engineering
Chen, Celestine Yi Ru
Engineering biofilms for efficient removal of arsenic from water
description One of the most fundamental necessity for mankind – clean water, is gradually decreasing as the world population increases beyond 7 billion. Arsenic contaminated groundwater is one of the main concerns, given its carcinogenic nature and widespread in waterbodies across the world. Various methods has been developed for arsenic removal, however, the application of these technologies is more often than not beyond the capacity of developing nations. In light of this, a research on the Shewanella oneidensis MR-1 strain was conducted to explore the feasibility of bacterial sorption of groundwater arsenic for the cleansing of water. S. oneidensis is a bacterium known for its functional protein, BpfA, which promotes the growth of biofilm. The innate ability of S. oneidensis to form biofilm for the sorption of arsenic is a more affordable method, however there is a threshold concentration limit to the extent of sorption. Hence, we explore the viability of modifying the genetics of the bacterium to enhance its sorption capacity. A mutant, BK3, was genetically-modified to express functional peptide for arsenic sorption on cell surface and was experimented in comparison with the wild type to differentiate their biofilm formation performance. In this report, the performance of wild type and BK3 in their biofilm and planktonic states will be discussed to determine the optimal conditions for sorption, and thus removal of arsenic.
author2 Cao Bin
author_facet Cao Bin
Chen, Celestine Yi Ru
format Final Year Project
author Chen, Celestine Yi Ru
author_sort Chen, Celestine Yi Ru
title Engineering biofilms for efficient removal of arsenic from water
title_short Engineering biofilms for efficient removal of arsenic from water
title_full Engineering biofilms for efficient removal of arsenic from water
title_fullStr Engineering biofilms for efficient removal of arsenic from water
title_full_unstemmed Engineering biofilms for efficient removal of arsenic from water
title_sort engineering biofilms for efficient removal of arsenic from water
publisher Nanyang Technological University
publishDate 2020
url https://hdl.handle.net/10356/140519
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