Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components

This paper describes the functionalization of solid supported phospholipid bilayer with decellularized extracellular matrix (dECM) components, toward the development of biomimetic platforms that more closely mimic the cell surface environment. The dECM was obtained through a combination of chemical...

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Main Authors: Vafaei, Setareh, Tabaei, Seyed Ruhollah, Guneta, Vipra, Choong, Cleo, Cho, Nam-Joon
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/141540
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1415402020-06-09T03:36:38Z Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components Vafaei, Setareh Tabaei, Seyed Ruhollah Guneta, Vipra Choong, Cleo Cho, Nam-Joon School of Chemical and Biomedical Engineering School of Materials Science and Engineering Centre for Biomimetic Sensor Science Engineering::Materials Functionalization Vesicles This paper describes the functionalization of solid supported phospholipid bilayer with decellularized extracellular matrix (dECM) components, toward the development of biomimetic platforms that more closely mimic the cell surface environment. The dECM was obtained through a combination of chemical and enzymatic treatments of mouse adipose tissue that contains collagen, fibronectin, and glycosaminoglycans (GAGs). Using amine coupling chemistry, the dECM components were attached covalently to the surface of a supported lipid bilayer containing phospholipids with reactive carboxylic acid headgroups. The bilayer formation and the kinetics of subsequent dECM conjugation were monitored by quartz crystal microbalance with dissipation (QCM-D). Fluorescence recovery after photobleaching (FRAP) confirmed the fluidity of the membrane after functionalization with dECM. The resulting hybrid biomimetic interface supports the attachment and survival of the human hepatocyte Huh 7.5 and maintains the representative hepatocellular function. Importantly, the platform is suitable for monitoring the lateral organization and clustering of cell-binding ligands and growth factor receptors in the presence of the rich biochemical profile of tissue-derived ECM components. NRF (Natl Research Foundation, S’pore) 2020-06-09T03:36:38Z 2020-06-09T03:36:38Z 2018 Journal Article Vafaei, S. Tabaei, S. R., Guneta, V., Choong, C., & Cho, N.-J. (2018). Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components. Langmuir, 34(11), 3507-3516. doi:10.1021/acs.langmuir.7b03265 0743-7463 https://hdl.handle.net/10356/141540 10.1021/acs.langmuir.7b03265 29489371 2-s2.0-85044205274 11 34 3507 3516 en Langmuir © 2018 American Chemical Society. All rights reserved.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Engineering::Materials
Functionalization
Vesicles
spellingShingle Engineering::Materials
Functionalization
Vesicles
Vafaei, Setareh
Tabaei, Seyed Ruhollah
Guneta, Vipra
Choong, Cleo
Cho, Nam-Joon
Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
description This paper describes the functionalization of solid supported phospholipid bilayer with decellularized extracellular matrix (dECM) components, toward the development of biomimetic platforms that more closely mimic the cell surface environment. The dECM was obtained through a combination of chemical and enzymatic treatments of mouse adipose tissue that contains collagen, fibronectin, and glycosaminoglycans (GAGs). Using amine coupling chemistry, the dECM components were attached covalently to the surface of a supported lipid bilayer containing phospholipids with reactive carboxylic acid headgroups. The bilayer formation and the kinetics of subsequent dECM conjugation were monitored by quartz crystal microbalance with dissipation (QCM-D). Fluorescence recovery after photobleaching (FRAP) confirmed the fluidity of the membrane after functionalization with dECM. The resulting hybrid biomimetic interface supports the attachment and survival of the human hepatocyte Huh 7.5 and maintains the representative hepatocellular function. Importantly, the platform is suitable for monitoring the lateral organization and clustering of cell-binding ligands and growth factor receptors in the presence of the rich biochemical profile of tissue-derived ECM components.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Vafaei, Setareh
Tabaei, Seyed Ruhollah
Guneta, Vipra
Choong, Cleo
Cho, Nam-Joon
format Article
author Vafaei, Setareh
Tabaei, Seyed Ruhollah
Guneta, Vipra
Choong, Cleo
Cho, Nam-Joon
author_sort Vafaei, Setareh
title Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
title_short Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
title_full Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
title_fullStr Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
title_full_unstemmed Hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
title_sort hybrid biomimetic interfaces integrating supported lipid bilayers with decellularized extracellular matrix components
publishDate 2020
url https://hdl.handle.net/10356/141540
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