ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages
Studies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental understanding of how proteins deform membrane. We previously showed that a BAR domain in tandem with a Pleckstrin Homology (PH domain) underlies the assembly of ACAP1 (Arfgap with Coil-coil, Ankryin repeat, and PH domain I)...
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sg-ntu-dr.10356-1420212023-02-28T17:07:20Z ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages Chan, Chun Pang, Xiaoyun Zhang, Yan Niu, Tongxin Yang, Shengjiang Zhao, Daohui Li, Jian Lu, Lanyuan Hsu, Victor W. Zhou, Jian Sun, Fei Fan, Jun School of Biological Sciences Science::Biological sciences Biochemical Simulations Dimers Studies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental understanding of how proteins deform membrane. We previously showed that a BAR domain in tandem with a Pleckstrin Homology (PH domain) underlies the assembly of ACAP1 (Arfgap with Coil-coil, Ankryin repeat, and PH domain I) into an unusual lattice structure that also uncovers a new paradigm for how a BAR protein deforms membrane. Here, we initially pursued computation-based refinement of the ACAP1 lattice to identify its critical protein contacts. Simulation studies then revealed how ACAP1, which dimerizes into a symmetrical structure in solution, is recruited asymmetrically to the membrane through dynamic behavior. We also pursued electron microscopy (EM)-based structural studies, which shed further insight into the dynamic nature of the ACAP1 lattice assembly. As ACAP1 is an unconventional BAR protein, our findings broaden the understanding of the mechanistic spectrum by which proteins assemble into higher-ordered structures to achieve membrane deformation. Published version 2020-06-15T02:56:52Z 2020-06-15T02:56:52Z 2019 Journal Article Chan, C., Pang, X., Zhang, Y., Niu, T., Yang, S., Zhao, D., . . . Fan, J. (2019). ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages. PLoS Computational Biology, 15(7), e1007081-. doi:10.1371/journal.pcbi.1007081 1553-734X https://hdl.handle.net/10356/142021 10.1371/journal.pcbi.1007081 31291238 2-s2.0-85070788968 7 15 en PLoS Computational Biology © 2019 Chan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
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Science::Biological sciences Biochemical Simulations Dimers Chan, Chun Pang, Xiaoyun Zhang, Yan Niu, Tongxin Yang, Shengjiang Zhao, Daohui Li, Jian Lu, Lanyuan Hsu, Victor W. Zhou, Jian Sun, Fei Fan, Jun ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
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Studies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental understanding of how proteins deform membrane. We previously showed that a BAR domain in tandem with a Pleckstrin Homology (PH domain) underlies the assembly of ACAP1 (Arfgap with Coil-coil, Ankryin repeat, and PH domain I) into an unusual lattice structure that also uncovers a new paradigm for how a BAR protein deforms membrane. Here, we initially pursued computation-based refinement of the ACAP1 lattice to identify its critical protein contacts. Simulation studies then revealed how ACAP1, which dimerizes into a symmetrical structure in solution, is recruited asymmetrically to the membrane through dynamic behavior. We also pursued electron microscopy (EM)-based structural studies, which shed further insight into the dynamic nature of the ACAP1 lattice assembly. As ACAP1 is an unconventional BAR protein, our findings broaden the understanding of the mechanistic spectrum by which proteins assemble into higher-ordered structures to achieve membrane deformation. |
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School of Biological Sciences |
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School of Biological Sciences Chan, Chun Pang, Xiaoyun Zhang, Yan Niu, Tongxin Yang, Shengjiang Zhao, Daohui Li, Jian Lu, Lanyuan Hsu, Victor W. Zhou, Jian Sun, Fei Fan, Jun |
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Article |
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Chan, Chun Pang, Xiaoyun Zhang, Yan Niu, Tongxin Yang, Shengjiang Zhao, Daohui Li, Jian Lu, Lanyuan Hsu, Victor W. Zhou, Jian Sun, Fei Fan, Jun |
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Chan, Chun |
title |
ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
title_short |
ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
title_full |
ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
title_fullStr |
ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
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ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
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acap1 assembles into an unusual protein lattice for membrane deformation through multiple stages |
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2020 |
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https://hdl.handle.net/10356/142021 |
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