Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery

Graphene quantum dots (GQDs) have been emerging as next‐generation bioimaging agents because of their intrinsic strong fluorescence, photostability, aqueous stability, biocompatibility, and facile synthesis. In this work, GQDs are encapsulated in ferritin protein nanocages to develop multi‐functiona...

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Main Authors: Nasrollahi, Fatemeh, Sana, Barindra, Paramelle, David, Ahadian, Samad, Khademhosseini, Ali, Lim, Sierin
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/142861
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1428612023-12-29T06:50:01Z Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery Nasrollahi, Fatemeh Sana, Barindra Paramelle, David Ahadian, Samad Khademhosseini, Ali Lim, Sierin School of Chemical and Biomedical Engineering p53 Laboratory, A*STAR NTU-Northwestern Institute for Nanomedicine Engineering::Chemical engineering Fluorescence Imaging Graphene Quantum Dot Graphene quantum dots (GQDs) have been emerging as next‐generation bioimaging agents because of their intrinsic strong fluorescence, photostability, aqueous stability, biocompatibility, and facile synthesis. In this work, GQDs are encapsulated in ferritin protein nanocages to develop multi‐functional nanoplatforms toward multi‐modal imaging and cancer therapy. Encapsulation of ultra‐small GQDs is expected to reduce their quick excretion from the body and increase their bioimaging efficiency. To expand the functionality of protein nanocages as multi‐modal imaging nanoprobes capable of both fluorescence and magnetic resonance imaging (MRI), GQDs and iron are encapsulated inside the core of AfFtn‐AA (an engineered ferritin nanocage derived from the archaeon Archaeoglobus fulgidus ). The co‐encapsulation is achieved through an iron‐mediated, self‐assembly of ferritin dimers resulting in the formation of GQD–iron complex in the ferritin nanocages ((GQDs/Fe)AA). The (GQDs/Fe)AA shows high relaxivities in MRI and pH‐sensitive fluorescence with strong fluorescence at low pH values and on MDA‐MB‐231 cells. As an imaging agent and a drug nanocarrier, (GQDs/Fe)AA exhibits negligible cytotoxicity on the cells and a high loading capacity (35%) of doxorubicin. Taken together, the (GQDs/Fe)AA shows promising applications in cancer diagnosis and therapy as a pH‐responsive fluorophore, MRI agent, and drug nanocarrier. Accepted version 2020-07-06T04:24:13Z 2020-07-06T04:24:13Z 2020 Journal Article Nasrollahi, F., Sana, B., Paramelle, D., Ahadian, S., Khademhosseini, A., & Lim, S. (2020). Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery. Advanced Therapeutics, 3(3), 1900183-. doi:10.1002/adtp.201900183 2366-3987 https://hdl.handle.net/10356/142861 10.1002/adtp.201900183 3 3 en Advanced Therapeutics This is the accepted version of the following article: Nasrollahi, F., Sana, B., Paramelle, D., Ahadian, S., Khademhosseini, A., & Lim, S. (2020). Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery. Advanced Therapeutics, 3(3), 1900183-, which has been published in final form at https://doi.org/10.1002/adtp.201900183. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
Fluorescence Imaging
Graphene Quantum Dot
spellingShingle Engineering::Chemical engineering
Fluorescence Imaging
Graphene Quantum Dot
Nasrollahi, Fatemeh
Sana, Barindra
Paramelle, David
Ahadian, Samad
Khademhosseini, Ali
Lim, Sierin
Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
description Graphene quantum dots (GQDs) have been emerging as next‐generation bioimaging agents because of their intrinsic strong fluorescence, photostability, aqueous stability, biocompatibility, and facile synthesis. In this work, GQDs are encapsulated in ferritin protein nanocages to develop multi‐functional nanoplatforms toward multi‐modal imaging and cancer therapy. Encapsulation of ultra‐small GQDs is expected to reduce their quick excretion from the body and increase their bioimaging efficiency. To expand the functionality of protein nanocages as multi‐modal imaging nanoprobes capable of both fluorescence and magnetic resonance imaging (MRI), GQDs and iron are encapsulated inside the core of AfFtn‐AA (an engineered ferritin nanocage derived from the archaeon Archaeoglobus fulgidus ). The co‐encapsulation is achieved through an iron‐mediated, self‐assembly of ferritin dimers resulting in the formation of GQD–iron complex in the ferritin nanocages ((GQDs/Fe)AA). The (GQDs/Fe)AA shows high relaxivities in MRI and pH‐sensitive fluorescence with strong fluorescence at low pH values and on MDA‐MB‐231 cells. As an imaging agent and a drug nanocarrier, (GQDs/Fe)AA exhibits negligible cytotoxicity on the cells and a high loading capacity (35%) of doxorubicin. Taken together, the (GQDs/Fe)AA shows promising applications in cancer diagnosis and therapy as a pH‐responsive fluorophore, MRI agent, and drug nanocarrier.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Nasrollahi, Fatemeh
Sana, Barindra
Paramelle, David
Ahadian, Samad
Khademhosseini, Ali
Lim, Sierin
format Article
author Nasrollahi, Fatemeh
Sana, Barindra
Paramelle, David
Ahadian, Samad
Khademhosseini, Ali
Lim, Sierin
author_sort Nasrollahi, Fatemeh
title Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
title_short Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
title_full Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
title_fullStr Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
title_full_unstemmed Incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
title_sort incorporation of graphene quantum dots, iron, and doxorubicin in/on ferritin nanocages for bimodal imaging and drug delivery
publishDate 2020
url https://hdl.handle.net/10356/142861
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