Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells

The pathogenesis of severe malaria revolves around the pathological changes within the vasculature during the infection. Infected erythrocytes (IRBC) can sequester within the microvasculature via phenomena such as IRBC-endothelial cytoadherence, rosetting, and platelet mediated agglutination of IRBC...

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Main Author: Xie, Viviana Xinjie
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Format: Final Year Project
Language:English
Published: Nanyang Technological University 2020
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Online Access:https://hdl.handle.net/10356/143297
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spelling sg-ntu-dr.10356-1432972023-02-28T18:07:54Z Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells Xie, Viviana Xinjie - School of Biological Sciences Singapore Immunology Network (SIgN), A*STAR Infectious Diseases Horizontal Technology Centre (ID HTC), A*STAR Laurent Rénia Wenn-Chyau Lee renia_laurent@immunol.astar.edu.sg, lee_wenn_chyau@immunol.astar.edu.sg Science::Biological sciences The pathogenesis of severe malaria revolves around the pathological changes within the vasculature during the infection. Infected erythrocytes (IRBC) can sequester within the microvasculature via phenomena such as IRBC-endothelial cytoadherence, rosetting, and platelet mediated agglutination of IRBC. This results in hampering of blood flow, giving rise to endothelial activation and injuries. Recently, insulin-like growth factor binding protein-7 (IGFBP7) has been reported to stimulate rosette formation of IRBC. As IGFBP7 has been associated with several vascular disorders, we decided to quantitate the IGFBP7 secretion by endothelial cells upon exposure to the parasite antigens so as to understand the microvasculature dynamics during malaria pathogenesis. We used human endothelial cell lines derived from microvasculature of brain, lungs and kidneys. The endothelial cells were exposed to culture supernatant of parasites Plasmodium falciparum (strain 3D7) and P. knowlesi (strain A1-H.1) separately for 24 hours prior to IGFBP7 quantification via ELISA performed on culture supernatant of the endothelial cells. We found that only the kidney-derived human renal glomerular endothelial cells (HGREC) that were exposed to P. knowlesi culture supernatant demonstrated significant increment in IGFBP7 secretion. This study shows that different endothelial cells react differently to different species of malaria parasites in the context of IGFBP7 secretion. Bachelor of Science in Biological Sciences 2020-08-19T08:29:43Z 2020-08-19T08:29:43Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/143297 en NMRC/OFYIRG/0070/2018 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Xie, Viviana Xinjie
Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
description The pathogenesis of severe malaria revolves around the pathological changes within the vasculature during the infection. Infected erythrocytes (IRBC) can sequester within the microvasculature via phenomena such as IRBC-endothelial cytoadherence, rosetting, and platelet mediated agglutination of IRBC. This results in hampering of blood flow, giving rise to endothelial activation and injuries. Recently, insulin-like growth factor binding protein-7 (IGFBP7) has been reported to stimulate rosette formation of IRBC. As IGFBP7 has been associated with several vascular disorders, we decided to quantitate the IGFBP7 secretion by endothelial cells upon exposure to the parasite antigens so as to understand the microvasculature dynamics during malaria pathogenesis. We used human endothelial cell lines derived from microvasculature of brain, lungs and kidneys. The endothelial cells were exposed to culture supernatant of parasites Plasmodium falciparum (strain 3D7) and P. knowlesi (strain A1-H.1) separately for 24 hours prior to IGFBP7 quantification via ELISA performed on culture supernatant of the endothelial cells. We found that only the kidney-derived human renal glomerular endothelial cells (HGREC) that were exposed to P. knowlesi culture supernatant demonstrated significant increment in IGFBP7 secretion. This study shows that different endothelial cells react differently to different species of malaria parasites in the context of IGFBP7 secretion.
author2 -
author_facet -
Xie, Viviana Xinjie
format Final Year Project
author Xie, Viviana Xinjie
author_sort Xie, Viviana Xinjie
title Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
title_short Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
title_full Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
title_fullStr Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
title_full_unstemmed Effect of exposure to malaria parasites on IGFBP7 expression profile of endothelial cells
title_sort effect of exposure to malaria parasites on igfbp7 expression profile of endothelial cells
publisher Nanyang Technological University
publishDate 2020
url https://hdl.handle.net/10356/143297
_version_ 1759854328351293440