Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects

Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whos...

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Main Authors: Awasthi, Kamlesh, Change, Feng-Lin, Hsu, Hsin-Yun, Hsieh, Pei-Ying, Ohta, Nobuhiro
Other Authors: Asian Spectroscopy Conference 2020
Format: Conference or Workshop Item
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/144287
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1442872020-10-29T20:11:04Z Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects Awasthi, Kamlesh Change, Feng-Lin Hsu, Hsin-Yun Hsieh, Pei-Ying Ohta, Nobuhiro Asian Spectroscopy Conference 2020 Institute of Advanced Studies Science::Chemistry Fluorescence Lifetime Spectroscopy Autofluorescence Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whose pulse width is shorter than the charging time of the outer membrane is applied to living systems, the pulsed electric field may penetrate into the cell and induce changes in intracellular function. In fact, we reported the apoptosis induced by application of nanosecond pulsed electric field (nsPEF) with a pulse width of 10–50 ns in HeLa cells.1 Published version 2020-10-27T02:15:57Z 2020-10-27T02:15:57Z 2020 Conference Paper Awasthi, K., Change, F.-L., Hsu, H.-Y., Hsieh, P.-Y., & Ohta, N. (2020). Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects. Proc. Of the 7th Asian Spectroscopy Conference (ASC 2020). doi:10.32655/ASC_8-10_Dec2020.40 https://hdl.handle.net/10356/144287 10.32655/ASC_8-10_Dec2020.40 en © 2020 Nanyang Technological University. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
Fluorescence Lifetime Spectroscopy
Autofluorescence
spellingShingle Science::Chemistry
Fluorescence Lifetime Spectroscopy
Autofluorescence
Awasthi, Kamlesh
Change, Feng-Lin
Hsu, Hsin-Yun
Hsieh, Pei-Ying
Ohta, Nobuhiro
Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
description Electric field effects on dynamics and function of biological systems depend on the cell membrane capacitance and on the pulse duration of the applied electric field, which enables selective application of pulsed electric field in specific organelles and specific living cells. If electric field whose pulse width is shorter than the charging time of the outer membrane is applied to living systems, the pulsed electric field may penetrate into the cell and induce changes in intracellular function. In fact, we reported the apoptosis induced by application of nanosecond pulsed electric field (nsPEF) with a pulse width of 10–50 ns in HeLa cells.1
author2 Asian Spectroscopy Conference 2020
author_facet Asian Spectroscopy Conference 2020
Awasthi, Kamlesh
Change, Feng-Lin
Hsu, Hsin-Yun
Hsieh, Pei-Ying
Ohta, Nobuhiro
format Conference or Workshop Item
author Awasthi, Kamlesh
Change, Feng-Lin
Hsu, Hsin-Yun
Hsieh, Pei-Ying
Ohta, Nobuhiro
author_sort Awasthi, Kamlesh
title Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
title_short Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
title_full Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
title_fullStr Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
title_full_unstemmed Autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
title_sort autofluorescence lifetime microscopy in normal and cancer cells and nanosecond pulsed electric field effects
publishDate 2020
url https://hdl.handle.net/10356/144287
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