Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells

• Main Authors: Vongsvivut, Jitraporn (Pimm), Pérez-Guaita, D., Wood, B. R., Heraud, P., Khambatta, K., Hartnell, D., Hackett, M. J., Tobin, M. J.
• Other Authors: Asian Spectroscopy Conference 2020
• Format: Conference or Workshop Item
• Language: English
• Published: 2020
• Subjects: Science::Chemistry; Synchrotron Infrared; ATR FTIR
• Online Access: https://hdl.handle.net/10356/144295
• Institution: Nanyang Technological University

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy has been used widely for probing the molecular properties of materials. Coupling a synchrotron infrared (IR) beam to an ATR element using a high numerical aperture (NA) microscope objective enhances the spatial resolution, relative to transmission or transflectance microspectroscopy, by a factor proportional to the refractive index (n) of the ATR element. This work presents the development of the synchrotron macro ATR-FTIR microspectroscopy at Australian Synchrotron Infrared Microspectroscopy (IRM) Beamline, and demonstrates that high quality FTIR chemical maps of single cells and tissues can be achieved at an enhanced spatial resolution [1].