Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus
Brassica napus is an important oilseed crop in the world and the mechanism of seed oil biosynthesis in B. napus remains unclear. In order to study the mechanism of oil biosynthesis and generate germplasms for breeding, an EMS mutant population with ~100,000 M2 lines was generated using Zhongshuang 1...
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sg-ntu-dr.10356-1449052023-02-28T17:08:56Z Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus Tang, Shan Liu, Dong-Xu Lu, Shaoping Yu, Liangqian Li, Yuqing Lin, Shengli Li, Long Du, Zhuolin Liu, Xiao Li, Xiao Ma, Wei Yang, Qing-Yong Guo, Liang School of Biological Sciences Science::Biological sciences Brassica Napus EMS Brassica napus is an important oilseed crop in the world and the mechanism of seed oil biosynthesis in B. napus remains unclear. In order to study the mechanism of oil biosynthesis and generate germplasms for breeding, an EMS mutant population with ~100,000 M2 lines was generated using Zhongshuang 11 as the parent line. The EMS-induced genome-wide mutations in M2-M4 plants were assessed. The average number of mutations including single nucleotide polymorphisms and insertion/deletion in M2-M4 was 21,177, 28,675 and 17,915, respectively. The effects of the mutations on gene function were predicted in M2-M4 mutants, respectively. We screened the seeds from 98,113 M2 lines and 9,415 seed oil content and fatty acid mutants were identified. We further confirmed 686 mutants with altered seed oil content and fatty acid in advanced generation (M4 seeds). Five representative M4 mutants with increased oleic acid were re-sequenced and the potential causal variations in FAD2 and ROD1 genes were identified. This study generated and screened a large scale of B. napus EMS mutant population and the identified mutants could provide useful genetic resources for the study of oil biosynthesis and genetic improvement of seed oil content and fatty acid composition of B. napus in the future. Accepted version This research was supported by The National Key Research and Development Program of China (2016YFD0101000) and the National Natural Science Foundation of China (31871658). 2020-12-03T01:07:18Z 2020-12-03T01:07:18Z 2020 Journal Article Tang, S., Liu, D.-X., Lu, S., Yu, L., Li, Y., Lin, S., ... Guo, L. (2020). Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus. Plant Journal, 104(5), 1410-1422. doi:10.1111/tpj.15003 1365-313X https://hdl.handle.net/10356/144905 10.1111/tpj.15003 33048384 5 104 1410 1422 en Plant Journal This is the accepted version of the following article: Tang, S., Liu, D.-X., Lu, S., Yu, L., Li, Y., Lin, S., ... Guo, L. (2020). Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus. Plant Journal, 104(5), 1410-1422. doi:10.1111/tpj.15003, which has been published in final form at http://dx.doi.org/10.1111/tpj.15003. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html]. application/pdf |
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Science::Biological sciences Brassica Napus EMS Tang, Shan Liu, Dong-Xu Lu, Shaoping Yu, Liangqian Li, Yuqing Lin, Shengli Li, Long Du, Zhuolin Liu, Xiao Li, Xiao Ma, Wei Yang, Qing-Yong Guo, Liang Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
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Brassica napus is an important oilseed crop in the world and the mechanism of seed oil biosynthesis in B. napus remains unclear. In order to study the mechanism of oil biosynthesis and generate germplasms for breeding, an EMS mutant population with ~100,000 M2 lines was generated using Zhongshuang 11 as the parent line. The EMS-induced genome-wide mutations in M2-M4 plants were assessed. The average number of mutations including single nucleotide polymorphisms and insertion/deletion in M2-M4 was 21,177, 28,675 and 17,915, respectively. The effects of the mutations on gene function were predicted in M2-M4 mutants, respectively. We screened the seeds from 98,113 M2 lines and 9,415 seed oil content and fatty acid mutants were identified. We further confirmed 686 mutants with altered seed oil content and fatty acid in advanced generation (M4 seeds). Five representative M4 mutants with increased oleic acid were re-sequenced and the potential causal variations in FAD2 and ROD1 genes were identified. This study generated and screened a large scale of B. napus EMS mutant population and the identified mutants could provide useful genetic resources for the study of oil biosynthesis and genetic improvement of seed oil content and fatty acid composition of B. napus in the future. |
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School of Biological Sciences |
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School of Biological Sciences Tang, Shan Liu, Dong-Xu Lu, Shaoping Yu, Liangqian Li, Yuqing Lin, Shengli Li, Long Du, Zhuolin Liu, Xiao Li, Xiao Ma, Wei Yang, Qing-Yong Guo, Liang |
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Article |
author |
Tang, Shan Liu, Dong-Xu Lu, Shaoping Yu, Liangqian Li, Yuqing Lin, Shengli Li, Long Du, Zhuolin Liu, Xiao Li, Xiao Ma, Wei Yang, Qing-Yong Guo, Liang |
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Tang, Shan |
title |
Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
title_short |
Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
title_full |
Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
title_fullStr |
Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
title_full_unstemmed |
Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus |
title_sort |
development and screening of ems mutants with altered seed oil content or fatty acid composition in brassica napus |
publishDate |
2020 |
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https://hdl.handle.net/10356/144905 |
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1759853519020490752 |