Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production
Cyanobacteria, which use solar energy to convert carbon dioxide into biomass, are potential solar biorefineries for the sustainable production of chemicals and biofuels. However, yields obtained with current strains are still uncompetitive compared to existing heterotrophic production systems. Here...
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sg-ntu-dr.10356-1453702023-02-28T16:56:58Z Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production Włodarczyk, Artur Selão, Tiago Toscano Norling, Birgitta Nixon, Peter Julian School of Biological Sciences Science::Biological sciences Marine Microbiology Metabolic Engineering Cyanobacteria, which use solar energy to convert carbon dioxide into biomass, are potential solar biorefineries for the sustainable production of chemicals and biofuels. However, yields obtained with current strains are still uncompetitive compared to existing heterotrophic production systems. Here we report the discovery and characterization of a new cyanobacterial strain, Synechococcus sp. PCC 11901, with promising features for green biotechnology. It is naturally transformable, has a short doubling time of ≈2 hours, grows at high light intensities and in a wide range of salinities and accumulates up to ≈33 g dry cell weight per litre when cultured in a shake-flask system using a modified growth medium − 1.7 to 3 times more than other strains tested under similar conditions. As a proof of principle, PCC 11901 engineered to produce free fatty acids yielded over 6 mM (1.5 g L−1), an amount comparable to that achieved by similarly engineered heterotrophic organisms.. Nanyang Technological University Published version This work was supported by NTU grants M4080306 to BN and M4081714 to P.J.N. We would like to thank Daniela Moses (SCELSE, NTU) for assistance with whole genome sequencing reactions, Anthony Wong (SCELSE, NTU) and the SBS Microscopy facility for assistance with fluorescence microscopy imaging, Giulia Mastroianni (School of Biological and Chemical Sciences, Queen Mary University of London) for TEM sample preparation and analysis, the SBS Flow Cytometry facility and Prof. Adriana Lopes dos Santos (ASE, NTU) for assistance with flow cytometry data analysis and Michael Voigtmann (Wintershine Pte. Ltd.) for providing access to the sample collection site. The authors are also grateful to Prof. Bertil Andersson for his constant support and encouragement and his pivotal role in establishing the CyanoSynBio@NTU laboratory. 2020-12-18T08:07:33Z 2020-12-18T08:07:33Z 2020 Journal Article Włodarczyk, A., Selão, T. T., Norling, B., & Nixon, P. J. (2020). Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production. Communications Biology, 3(1), 215-. doi:10.1038/s42003-020-0910-8 2399-3642 https://hdl.handle.net/10356/145370 10.1038/s42003-020-0910-8 32382027 1 3 en M4080306 M4081714 Communications Biology © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
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Science::Biological sciences Marine Microbiology Metabolic Engineering Włodarczyk, Artur Selão, Tiago Toscano Norling, Birgitta Nixon, Peter Julian Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
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Cyanobacteria, which use solar energy to convert carbon dioxide into biomass, are potential solar biorefineries for the sustainable production of chemicals and biofuels. However, yields obtained with current strains are still uncompetitive compared to existing heterotrophic production systems. Here we report the discovery and characterization of a new cyanobacterial strain, Synechococcus sp. PCC 11901, with promising features for green biotechnology. It is naturally transformable, has a short doubling time of ≈2 hours, grows at high light intensities and in a wide range of salinities and accumulates up to ≈33 g dry cell weight per litre when cultured in a shake-flask system using a modified growth medium − 1.7 to 3 times more than other strains tested under similar conditions. As a proof of principle, PCC 11901 engineered to produce free fatty acids yielded over 6 mM (1.5 g L−1), an amount comparable to that achieved by similarly engineered heterotrophic organisms.. |
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School of Biological Sciences |
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School of Biological Sciences Włodarczyk, Artur Selão, Tiago Toscano Norling, Birgitta Nixon, Peter Julian |
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Article |
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Włodarczyk, Artur Selão, Tiago Toscano Norling, Birgitta Nixon, Peter Julian |
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Włodarczyk, Artur |
title |
Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
title_short |
Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
title_full |
Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
title_fullStr |
Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
title_full_unstemmed |
Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production |
title_sort |
newly discovered synechococcus sp. pcc 11901 is a robust cyanobacterial strain for high biomass production |
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2020 |
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https://hdl.handle.net/10356/145370 |
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1759856260096720896 |