Evaluation of droplet digital polymerase chain reaction (ddPCR) for the absolute quantification of Aspergillus species in the human airway

• Main Authors: Poh, Tuang Yeow, Nur A’tikah Mohamed Ali, Chan, Louisa L. Y., Tiew, Pei Yee, Chotirmall, Sanjay Haresh
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; Aspergillus Fumigatus; Aspergillus Terreus
• Online Access: https://hdl.handle.net/10356/145726
• Institution: Nanyang Technological University

Background: Prior studies illustrate the presence and clinical importance of detecting Aspergillus species in the airways of patients with chronic respiratory disease. Despite this, a low fungal biomass and the presence of PCR inhibitors limits the usefulness of quantitative PCR (qPCR) for accurate...