Scaffold-based delivery of CRISPR/Cas9 ribonucleoproteins for genome editing

• Main Authors: Chooi, Wai Hon, Chin, Jiah Shin, Chew, Sing Yian
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2021
• Subjects: Engineering::Chemical engineering; Gene delivery; Electrospinning
• Online Access: https://hdl.handle.net/10356/145891
• Institution: Nanyang Technological University

The simple and versatile CRISPR/Cas9 system is a promising strategy for genome editing in mammalian cells. Generally, the genome editing components, namely Cas9 protein and single-guide RNA (sgRNA), are delivered in the format of plasmids, mRNA or ribonucleoprotein (RNP) complexes. In particular, no...