Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays

Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One...

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Main Authors: Tan, Li Kiang, Wong, Wing Yan, Yang, Hui Ting, Huber, Roland G., Bond, Peter J., Ng, Lee Ching, Maurer-Stroh, Sebastian, Hapuarachchi, Hapuarachchige Chanditha
Other Authors: School of Biological Sciences
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Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/145899
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1458992023-02-28T16:57:13Z Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays Tan, Li Kiang Wong, Wing Yan Yang, Hui Ting Huber, Roland G. Bond, Peter J. Ng, Lee Ching Maurer-Stroh, Sebastian Hapuarachchi, Hapuarachchige Chanditha School of Biological Sciences Science::Medicine Flavivirus Diagnostics Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate. Agency for Science, Technology and Research (A*STAR) National Environmental Agency (NEA) Published version This study was supported by the internal funds from the National Environment Agency (NEA), Singapore. S.M.-S. was partially supported by the A*STAR HEIDI programme (grant number: H1699f0013). 2021-01-14T01:36:03Z 2021-01-14T01:36:03Z 2019 Journal Article Tan, L. K., Wong, W. Y., Yang, H. T., Huber, R. G., Bond, P. J., Ng, L. C., . . . Hapuarachchi, H. C. (2019). Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays . Diagnostics, 10(1), 11-. doi:10.3390/diagnostics10010011 2075-4418 https://hdl.handle.net/10356/145899 10.3390/diagnostics10010011 31878299 2-s2.0-85078409012 1 10 en H1699f0013 Diagnostics © 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Flavivirus
Diagnostics
spellingShingle Science::Medicine
Flavivirus
Diagnostics
Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
description Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
format Article
author Tan, Li Kiang
Wong, Wing Yan
Yang, Hui Ting
Huber, Roland G.
Bond, Peter J.
Ng, Lee Ching
Maurer-Stroh, Sebastian
Hapuarachchi, Hapuarachchige Chanditha
author_sort Tan, Li Kiang
title Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
title_short Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
title_full Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
title_fullStr Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
title_full_unstemmed Flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
title_sort flavivirus cross-reactivity to dengue nonstructural protein 1 antigen detection assays
publishDate 2021
url https://hdl.handle.net/10356/145899
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