Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea
The Baltic Sea represents one of the largest brackish ecosystems where various environmental factors control dynamic seasonal shifts in the structure, diversity, and function of the planktonic microbial communities. In this study, despite seasonal fluctuations, several bacterial populations (<2%...
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sg-ntu-dr.10356-1459052023-03-29T01:15:50Z Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea Poorna, Weerarathna Vidanage Ko, Seok-Oh Oh, Seungdae School of Civil and Environmental Engineering Engineering::Civil engineering Baltic Sea Brackish Water The Baltic Sea represents one of the largest brackish ecosystems where various environmental factors control dynamic seasonal shifts in the structure, diversity, and function of the planktonic microbial communities. In this study, despite seasonal fluctuations, several bacterial populations (<2% of the total OTUs) that are highly dominant (25% of relative abundance) and highly frequently occurring (>85% of occurrence) over four seasons were identified. Mathematical models using occurrence frequency and relative abundance data were able to describe community assembly persisting over time. Further, this work uncovered one of the core bacterial populations phylogenetically affiliated to SAR11 subclade IIIa. The analysis of the hypervariable region of 16S rRNA gene and single copy housekeeping genes recovered from metagenomic datasets suggested that the population was unexpectedly evolutionarily closely related to those inhabiting a mesosaline lacustrine ecosystem rather than other marine/coastal members. Our metagenomic results further revealed that the newly-identified population was the major driver facilitating the seasonal shifts in the overall community structure over the brackish waters of the Baltic Sea. The core community uncovered in this study supports the presence of a brackish water microbiome distinguishable from other marine and freshwater counterparts and will be a useful sentinel for monitoring local/global environmental changes posed on brackish surface waters. Nanyang Technological University Published version Poorna Weerarathna Vidanage was supported by a start-up grant (to Seungdae Oh) and NTU research scholarship from Nanyang Technological University. 2021-01-14T02:31:28Z 2021-01-14T02:31:28Z 2020 Journal Article Poorna, W. V., Ko, S. & Oh, S. (2020). Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea. Water, 12(2). https://dx.doi.org/10.3390/w12020501 2073-4441 0000-0001-6013-2345 https://hdl.handle.net/10356/145905 10.3390/w12020501 2-s2.0-85081591275 2 12 en Water © 2020 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf |
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Engineering::Civil engineering Baltic Sea Brackish Water Poorna, Weerarathna Vidanage Ko, Seok-Oh Oh, Seungdae Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
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The Baltic Sea represents one of the largest brackish ecosystems where various environmental factors control dynamic seasonal shifts in the structure, diversity, and function of the planktonic microbial communities. In this study, despite seasonal fluctuations, several bacterial populations (<2% of the total OTUs) that are highly dominant (25% of relative abundance) and highly frequently occurring (>85% of occurrence) over four seasons were identified. Mathematical models using occurrence frequency and relative abundance data were able to describe community assembly persisting over time. Further, this work uncovered one of the core bacterial populations phylogenetically affiliated to SAR11 subclade IIIa. The analysis of the hypervariable region of 16S rRNA gene and single copy housekeeping genes recovered from metagenomic datasets suggested that the population was unexpectedly evolutionarily closely related to those inhabiting a mesosaline lacustrine ecosystem rather than other marine/coastal members. Our metagenomic results further revealed that the newly-identified population was the major driver facilitating the seasonal shifts in the overall community structure over the brackish waters of the Baltic Sea. The core community uncovered in this study supports the presence of a brackish water microbiome distinguishable from other marine and freshwater counterparts and will be a useful sentinel for monitoring local/global environmental changes posed on brackish surface waters. |
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School of Civil and Environmental Engineering |
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School of Civil and Environmental Engineering Poorna, Weerarathna Vidanage Ko, Seok-Oh Oh, Seungdae |
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Article |
author |
Poorna, Weerarathna Vidanage Ko, Seok-Oh Oh, Seungdae |
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Poorna, Weerarathna Vidanage |
title |
Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
title_short |
Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
title_full |
Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
title_fullStr |
Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
title_full_unstemmed |
Metagenomics uncovers a core SAR11 population in brackish surface waters of the Baltic Sea |
title_sort |
metagenomics uncovers a core sar11 population in brackish surface waters of the baltic sea |
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2021 |
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https://hdl.handle.net/10356/145905 |
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1761781408990232576 |