Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler

• Main Authors: Wee, Soon Keong, Sivalingam, Suppiah Paramalingam, Yap, Eric Peng Huat
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Medicine; Point-of-care; Diagnostics
• Online Access: https://hdl.handle.net/10356/145932
• Institution: Nanyang Technological University

There is an ongoing worldwide coronavirus disease 2019 (Covid-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At present, confirmatory diagnosis is by reverse transcription polymerase chain reaction (RT-PCR), typically taking several hours and requiring a molecul...