Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model
Clinical evaluation of skin lesions requires precise and reproducible technologies for their qualitative and quantitative assessment. In this study, we investigate the applicability of a custom-built dermatologic OCT system for longitudinal assessment of intradermal volumes in a mouse model. The OCT...
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sg-ntu-dr.10356-1461692023-12-29T06:50:20Z Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model Schuetzenberger, Kornelia Pfister, Martin Messner, Alina Garhöfer, Gerhard Hohenadl, Christine Pfeiffenberger, Ulrike Schmetterer, Leopold Werkmeister, René M. School of Chemical and Biomedical Engineering Science::Medicine Preclinical Research Translational Research Clinical evaluation of skin lesions requires precise and reproducible technologies for their qualitative and quantitative assessment. In this study, we investigate the applicability of a custom-built dermatologic OCT system for longitudinal assessment of intradermal volumes in a mouse model. The OCT, based on an akinetic swept laser working at 1310 nm was employed for visualization and quantification of intradermal deposits of three different hyaluronic acid-based hydrogel formulations – one commercial and two test substances. Hydrogels were applied in 22 BALB/c mice, and measurements were performed over a six-month time period. All hydrogels increased in volume within the first weeks and degraded steadily thereafter. The half-lifes of the test hydrogels (27.2 ± 13.6 weeks for Hydrogel 1, 31.5 ± 17.2 weeks for Hydrogel 2) were higher in comparison to the commercially available HA hydrogel (21.4 ± 12.0 weeks), although differences were not significant. The sphericity parameter was used for evaluation of the deposit geometry. While on the injection day the sphericities were similar (~0.75 ± 0.04), at later time points significant differences between the different test substances were found (T24: PRV 0.59 ± 0.09, Hydrogel 1 0.70 ± 0.11, Hydrogel 2 0.78 ± 0.07; p ≤ 0.012 for all pairs). This study shows the applicability of OCT imaging for quantitative assessment of the volumetric behavior of intradermal deposits in vivo. Published version 2021-01-28T09:25:10Z 2021-01-28T09:25:10Z 2020 Journal Article Schuetzenberger, K., Pfister, M., Messner, A., Garhöfer, G., Hohenadl, C., Pfeiffenberger, U., . . . Werkmeister, R. M. (2020). Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model. Scientific Reports, 10(1), 4245-. doi:10.1038/s41598-020-61276-9 2045-2322 0000-0002-0943-4644 https://hdl.handle.net/10356/146169 10.1038/s41598-020-61276-9 32144359 2-s2.0-85081380301 1 10 en Scientific Reports © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
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Science::Medicine Preclinical Research Translational Research Schuetzenberger, Kornelia Pfister, Martin Messner, Alina Garhöfer, Gerhard Hohenadl, Christine Pfeiffenberger, Ulrike Schmetterer, Leopold Werkmeister, René M. Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
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Clinical evaluation of skin lesions requires precise and reproducible technologies for their qualitative and quantitative assessment. In this study, we investigate the applicability of a custom-built dermatologic OCT system for longitudinal assessment of intradermal volumes in a mouse model. The OCT, based on an akinetic swept laser working at 1310 nm was employed for visualization and quantification of intradermal deposits of three different hyaluronic acid-based hydrogel formulations – one commercial and two test substances. Hydrogels were applied in 22 BALB/c mice, and measurements were performed over a six-month time period. All hydrogels increased in volume within the first weeks and degraded steadily thereafter. The half-lifes of the test hydrogels (27.2 ± 13.6 weeks for Hydrogel 1, 31.5 ± 17.2 weeks for Hydrogel 2) were higher in comparison to the commercially available HA hydrogel (21.4 ± 12.0 weeks), although differences were not significant. The sphericity parameter was used for evaluation of the deposit geometry. While on the injection day the sphericities were similar (~0.75 ± 0.04), at later time points significant differences between the different test substances were found (T24: PRV 0.59 ± 0.09, Hydrogel 1 0.70 ± 0.11, Hydrogel 2 0.78 ± 0.07; p ≤ 0.012 for all pairs). This study shows the applicability of OCT imaging for quantitative assessment of the volumetric behavior of intradermal deposits in vivo. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Schuetzenberger, Kornelia Pfister, Martin Messner, Alina Garhöfer, Gerhard Hohenadl, Christine Pfeiffenberger, Ulrike Schmetterer, Leopold Werkmeister, René M. |
format |
Article |
author |
Schuetzenberger, Kornelia Pfister, Martin Messner, Alina Garhöfer, Gerhard Hohenadl, Christine Pfeiffenberger, Ulrike Schmetterer, Leopold Werkmeister, René M. |
author_sort |
Schuetzenberger, Kornelia |
title |
Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
title_short |
Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
title_full |
Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
title_fullStr |
Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
title_full_unstemmed |
Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
title_sort |
cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/146169 |
_version_ |
1787136643895918592 |