Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing

Background: The fast-spreading of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli (ESBL-producing E. coli) and ESBL genes has become a big challenge to public health. The risk of spreading ESBL genes and pathogens in the environment and community has raised public health concern. The cha...

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Main Authors: Zhong, Yang, Guo, Siyao, Seow, Kelyn Lee Ghee, Ming, Glendon Ong Hong, Schlundt, Joergen
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/146409
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1464092023-12-29T06:47:51Z Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing Zhong, Yang Guo, Siyao Seow, Kelyn Lee Ghee Ming, Glendon Ong Hong Schlundt, Joergen School of Chemical and Biomedical Engineering NTU Food Technology Centre Engineering::Chemical engineering ESBL Singapore Background: The fast-spreading of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli (ESBL-producing E. coli) and ESBL genes has become a big challenge to public health. The risk of spreading ESBL genes and pathogens in the environment and community has raised public health concern. The characterizing and whole-genome sequencing studies of ESBL-producing bacteria from reservoir water in Singapore is still limited. Materials and methods: The reservoir water sample was taken from two randomly selected sampling points of the Chinese Garden (Jurong river reservoir), which is a popular reservoir park in Singapore. The bacteria of the water sample were collected with 0.45 µm filter membranes and enriched before processing with ESBL-producing E. coli screening. The collected ESBL positive isolates were further characterized by both phenotypic tests including disc diffusion and microdilution Minimum Inhibitory Concentration (MIC) test, and also genotypic test as whole-genome sequencing analysis. Besides, to investigate the transferability of the resistance gene, a conjugation test was performed with the J53 E. coli strain as the gene receptor. Result: Nine ESBL-producing E. coli isolates were collected and confirmed as ESBL-producing with both phenotypic and genotypic tests. A potential pathogen as ST131 clade A isolate was identified, and all isolates were determined to harbor a blaCTX-M gene. Among them, strain J1E4 was resistant to polymyxin E and confirmed to harboring a conjugatable mcr-1 gene. Further genetic environment analysis has reflected a conversed gene cluster formed by insert sequence (IS), blaCTX-M-15, and WbuC family cupin-fold metalloprotein, which may potentially jump from the plasmids to the chromosome. Conclusion: The first time we reported the whole genome sequencing (WGS) data of ESBL-producing E. coli including potential pathogen (ST131) present in reservoir water in Singapore. The ESBL-producing E. coli from reservoir water also carrying conjugatable colistin resistance genes which may become a risk to human health. Nanyang Technological University Published version This research was supported by Nanyang Technological University, Singapore. 2021-02-16T05:58:20Z 2021-02-16T05:58:20Z 2021 Journal Article Zhong, Y., Guo, S., Seow, K. L. G., Ming, G. O. H., & Schlundt, J. (2021). Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates from Jurong Lake, Singapore with Whole-Genome-Sequencing. International Journal of Environmental Research and Public Health, 18(3), 937-. doi:10.3390/ijerph18030937 1660-4601 https://hdl.handle.net/10356/146409 10.3390/ijerph18030937 33499011 2-s2.0-85099681325 3 18 en International journal of environmental research and public health © 2021 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
ESBL
Singapore
spellingShingle Engineering::Chemical engineering
ESBL
Singapore
Zhong, Yang
Guo, Siyao
Seow, Kelyn Lee Ghee
Ming, Glendon Ong Hong
Schlundt, Joergen
Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
description Background: The fast-spreading of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli (ESBL-producing E. coli) and ESBL genes has become a big challenge to public health. The risk of spreading ESBL genes and pathogens in the environment and community has raised public health concern. The characterizing and whole-genome sequencing studies of ESBL-producing bacteria from reservoir water in Singapore is still limited. Materials and methods: The reservoir water sample was taken from two randomly selected sampling points of the Chinese Garden (Jurong river reservoir), which is a popular reservoir park in Singapore. The bacteria of the water sample were collected with 0.45 µm filter membranes and enriched before processing with ESBL-producing E. coli screening. The collected ESBL positive isolates were further characterized by both phenotypic tests including disc diffusion and microdilution Minimum Inhibitory Concentration (MIC) test, and also genotypic test as whole-genome sequencing analysis. Besides, to investigate the transferability of the resistance gene, a conjugation test was performed with the J53 E. coli strain as the gene receptor. Result: Nine ESBL-producing E. coli isolates were collected and confirmed as ESBL-producing with both phenotypic and genotypic tests. A potential pathogen as ST131 clade A isolate was identified, and all isolates were determined to harbor a blaCTX-M gene. Among them, strain J1E4 was resistant to polymyxin E and confirmed to harboring a conjugatable mcr-1 gene. Further genetic environment analysis has reflected a conversed gene cluster formed by insert sequence (IS), blaCTX-M-15, and WbuC family cupin-fold metalloprotein, which may potentially jump from the plasmids to the chromosome. Conclusion: The first time we reported the whole genome sequencing (WGS) data of ESBL-producing E. coli including potential pathogen (ST131) present in reservoir water in Singapore. The ESBL-producing E. coli from reservoir water also carrying conjugatable colistin resistance genes which may become a risk to human health.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Zhong, Yang
Guo, Siyao
Seow, Kelyn Lee Ghee
Ming, Glendon Ong Hong
Schlundt, Joergen
format Article
author Zhong, Yang
Guo, Siyao
Seow, Kelyn Lee Ghee
Ming, Glendon Ong Hong
Schlundt, Joergen
author_sort Zhong, Yang
title Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
title_short Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
title_full Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
title_fullStr Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
title_full_unstemmed Characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from Jurong Lake, Singapore with whole-genome-sequencing
title_sort characterization of extended-spectrum beta-lactamase-producing escherichia coli isolates from jurong lake, singapore with whole-genome-sequencing
publishDate 2021
url https://hdl.handle.net/10356/146409
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