Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm

Photoacoustic imaging (PAI) is a rapidly growing imaging modality which offers the advantages of good optical contrast and high ultrasound resolution. Although PAI provides imaging depth beyond the optical diffusion limit, penetration depth in biological samples is limited due to absorption and scat...

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Main Authors: Sharma, Arunima, Srishti, Srishti, Periyasamy, Vijitha, Pramanik, Manojit
Other Authors: School of Chemical and Biomedical Engineering
Format: Conference or Workshop Item
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/146649
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1466492023-12-29T06:44:09Z Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm Sharma, Arunima Srishti, Srishti Periyasamy, Vijitha Pramanik, Manojit School of Chemical and Biomedical Engineering SPIE - Photons Plus Ultrasound: Imaging and Sensing 2020 Engineering::Bioengineering Photoacoustic Imaging Imaging Depth Photoacoustic imaging (PAI) is a rapidly growing imaging modality which offers the advantages of good optical contrast and high ultrasound resolution. Although PAI provides imaging depth beyond the optical diffusion limit, penetration depth in biological samples is limited due to absorption and scattering of light in tissues. Improvement in imaging depth has been achieved by irradiating the sample with laser pulses of near infrared-I (NIR-I) region (700 nm-900 nm) due to decreased scattering of light in tissues within this optical window. Recently, further improvement in imaging depth has been reported by irradiating the sample in near infrared-II (NIR-II) region (900 nm-1700 nm). In this work, imaging depth in breast tissues when samples were irradiated by wavelengths in different optical windows has been compared. Initially, Monte Carlo simulation for light propagation in biological tissues was performed to compute imaging depth for excitation wavelengths of 532 nm, 800 nm, and 1064 nm. Further, photoacoustic tomography at 532 nm, 740 nm, and 1064 nm and acoustic resolution photoacoustic microscopy at 570 nm and 1064 nm were conducted to validate the results. We have shown that maximum imaging depth is achieved by NIR-I (740 nm/800 nm) when surface energy for all wavelengths is kept constant. However, when the energy density is proportional to maximum permissible exposure (MPE) at corresponding wavelength, maximum imaging depth is achieved by 1064 nm (NIR-II window). Therefore, we conclude that increased MPE in NIR-II window is responsible for the improved penetration depth in breast tissue in this region. National Medical Research Council (NMRC) Accepted version The authors would like to thank Singapore Ministry of Health’s National Medical Research Council (No. NMRC/OFIRG/0005/2016:M4062012) for funding this research. Srishti would also like to thank NTU-India Connect Research program for providing her an opportunity to visit NTU, Singapore. The authors have no relevant financial interest in the manuscript and no other potential conflicts of interest to disclose. 2021-03-04T04:59:29Z 2021-03-04T04:59:29Z 2020 Conference Paper Sharma, A., Srishti, S., Periyasamy, V., & Pramanik, M. (2020). Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm. Proceedings of SPIE - Photons Plus Ultrasound: Imaging and Sensing 2020, 11240, 145-. doi:10.1117/12.2544568 9781510632431 https://hdl.handle.net/10356/146649 10.1117/12.2544568 2-s2.0-85082728445 11240 145 en Copyright 2020 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Bioengineering
Photoacoustic Imaging
Imaging Depth
spellingShingle Engineering::Bioengineering
Photoacoustic Imaging
Imaging Depth
Sharma, Arunima
Srishti, Srishti
Periyasamy, Vijitha
Pramanik, Manojit
Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
description Photoacoustic imaging (PAI) is a rapidly growing imaging modality which offers the advantages of good optical contrast and high ultrasound resolution. Although PAI provides imaging depth beyond the optical diffusion limit, penetration depth in biological samples is limited due to absorption and scattering of light in tissues. Improvement in imaging depth has been achieved by irradiating the sample with laser pulses of near infrared-I (NIR-I) region (700 nm-900 nm) due to decreased scattering of light in tissues within this optical window. Recently, further improvement in imaging depth has been reported by irradiating the sample in near infrared-II (NIR-II) region (900 nm-1700 nm). In this work, imaging depth in breast tissues when samples were irradiated by wavelengths in different optical windows has been compared. Initially, Monte Carlo simulation for light propagation in biological tissues was performed to compute imaging depth for excitation wavelengths of 532 nm, 800 nm, and 1064 nm. Further, photoacoustic tomography at 532 nm, 740 nm, and 1064 nm and acoustic resolution photoacoustic microscopy at 570 nm and 1064 nm were conducted to validate the results. We have shown that maximum imaging depth is achieved by NIR-I (740 nm/800 nm) when surface energy for all wavelengths is kept constant. However, when the energy density is proportional to maximum permissible exposure (MPE) at corresponding wavelength, maximum imaging depth is achieved by 1064 nm (NIR-II window). Therefore, we conclude that increased MPE in NIR-II window is responsible for the improved penetration depth in breast tissue in this region.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Sharma, Arunima
Srishti, Srishti
Periyasamy, Vijitha
Pramanik, Manojit
format Conference or Workshop Item
author Sharma, Arunima
Srishti, Srishti
Periyasamy, Vijitha
Pramanik, Manojit
author_sort Sharma, Arunima
title Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
title_short Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
title_full Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
title_fullStr Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
title_full_unstemmed Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
title_sort monte carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm
publishDate 2021
url https://hdl.handle.net/10356/146649
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