Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources

Cyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater using oxygenic photosynthesis to fix carbon dioxide into biomass. Their other major nutritional requirements for efficient g...

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Main Authors: Selão, Tiago Toscano, Włodarczyk, Artur, Nixon, Peter J., Norling, Birgitta
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/147058
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1470582023-02-28T17:08:04Z Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources Selão, Tiago Toscano Włodarczyk, Artur Nixon, Peter J. Norling, Birgitta School of Biological Sciences Science Cyanobacteria Laboratory Evolution Cyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater using oxygenic photosynthesis to fix carbon dioxide into biomass. Their other major nutritional requirements for efficient growth are sources of nitrogen (N) and phosphorus (P). As these organisms are more economically cultivated in outdoor open systems, there is a need to develop cost-effective approaches to prevent the growth of contaminating organisms, especially as the use of antibiotic selection markers is neither economically feasible nor ecologically desirable due to the risk of horizontal gene transfer. Here we have introduced a synthetic melamine degradation pathway into Syn7002 and evolved the resulting strain to efficiently use the nitrogen-rich xenobiotic compound melamine as the sole N source. We also show that expression of phosphite dehydrogenase in the absence of its cognate phosphite transporter permits growth of Syn7002 on phosphite and can be used as a selectable marker in Syn7002. We combined these two strategies to generate a strain that can grow on melamine and phosphite as sole N and P sources, respectively. This strain is able to resist deliberate contamination in large excess and should be a useful chassis for metabolic engineering and biotechnological applications using cyanobacteria. Nanyang Technological University Accepted version This work was supported by NTU grants M4080306 to BN and M4081714 to PJN. The authors would like to thank Daniela Moses (SCELCE, NTU) for assistance with whole genome sequencing reactions and the NTU Phenomics Centre for LC-MS/MS analysis of culture supernatants. 2021-03-19T07:36:21Z 2021-03-19T07:36:21Z 2019 Journal Article Selão, T. T., Włodarczyk, A., Nixon, P. J. & Norling, B. (2019). Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources. Metabolic Engineering, 54, 255-263. https://dx.doi.org/10.1016/j.ymben.2019.04.013 1096-7176 https://hdl.handle.net/10356/147058 10.1016/j.ymben.2019.04.013 31063791 2-s2.0-85065417968 54 255 263 en Metabolic Engineering © 2019 International Metabolic Engineering Society. Published by Elsevier Inc.This paper was published in Metabolic Engineering and is made available with permission of International Metabolic Engineering Society. Published by Elsevier Inc. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science
Cyanobacteria
Laboratory Evolution
spellingShingle Science
Cyanobacteria
Laboratory Evolution
Selão, Tiago Toscano
Włodarczyk, Artur
Nixon, Peter J.
Norling, Birgitta
Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
description Cyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater using oxygenic photosynthesis to fix carbon dioxide into biomass. Their other major nutritional requirements for efficient growth are sources of nitrogen (N) and phosphorus (P). As these organisms are more economically cultivated in outdoor open systems, there is a need to develop cost-effective approaches to prevent the growth of contaminating organisms, especially as the use of antibiotic selection markers is neither economically feasible nor ecologically desirable due to the risk of horizontal gene transfer. Here we have introduced a synthetic melamine degradation pathway into Syn7002 and evolved the resulting strain to efficiently use the nitrogen-rich xenobiotic compound melamine as the sole N source. We also show that expression of phosphite dehydrogenase in the absence of its cognate phosphite transporter permits growth of Syn7002 on phosphite and can be used as a selectable marker in Syn7002. We combined these two strategies to generate a strain that can grow on melamine and phosphite as sole N and P sources, respectively. This strain is able to resist deliberate contamination in large excess and should be a useful chassis for metabolic engineering and biotechnological applications using cyanobacteria.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Selão, Tiago Toscano
Włodarczyk, Artur
Nixon, Peter J.
Norling, Birgitta
format Article
author Selão, Tiago Toscano
Włodarczyk, Artur
Nixon, Peter J.
Norling, Birgitta
author_sort Selão, Tiago Toscano
title Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
title_short Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
title_full Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
title_fullStr Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
title_full_unstemmed Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources
title_sort growth and selection of the cyanobacterium synechococcus sp. pcc 7002 using alternative nitrogen and phosphorus sources
publishDate 2021
url https://hdl.handle.net/10356/147058
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