Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803
Thylakoid biogenesis is an intricate process requiring accurate and timely assembly of proteins, pigments and other cofactors into functional, photosynthetically competent membranes. PSII assembly is studied in particular as its core protein, D1, is very susceptible to photodamage and has a high tur...
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sg-ntu-dr.10356-1470602023-02-28T17:08:05Z Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 Selão, Tiago Toscano Zhang, Lifang Knoppová, Jana Komenda, Josef Norling, Birgitta School of Biological Sciences Science Cyanobacteria Thylakoid Biogenesis Thylakoid biogenesis is an intricate process requiring accurate and timely assembly of proteins, pigments and other cofactors into functional, photosynthetically competent membranes. PSII assembly is studied in particular as its core protein, D1, is very susceptible to photodamage and has a high turnover rate, particularly in high light. PSII assembly is a modular process, with assembly steps proceeding in a specific order. Using aqueous two-phase partitioning to separate plasma membranes (PM) and thylakoid membranes (TM), we studied the subcellular localization of the early assembly steps for PSII biogenesis in a Synechocystis sp. PCC6803 cyanobacterium strain lacking the CP47 antenna. This strain accumulates the early D1-D2 assembly complex which was localized in TM along with associated PSII assembly factors. We also followed insertion and processing of the D1 precursor (pD1) by radioactive pulse-chase labeling. D1 is inserted into the membrane with a C-terminal extension which requires cleavage by a specific protease, the C-terminal processing protease (CtpA), to allow subsequent assembly of the oxygen-evolving complex. pD1 insertion as well as its conversion to mature D1 under various light conditions was seen only in the TM. Epitope-tagged CtpA was also localized in the same membrane, providing further support for the thylakoid location of pD1 processing. However, Vipp1 and PratA, two proteins suggested to be part of the so-called 'thylakoid centers', were found to associate with the PM. Together, these results suggest that early PSII assembly steps occur in TM or specific areas derived from them, with interaction with PM needed for efficient PSII and thylakoid biogenesis. Accepted version 2021-03-19T07:58:48Z 2021-03-19T07:58:48Z 2016 Journal Article Selão, T. T., Zhang, L., Knoppová, J., Komenda, J. & Norling, B. (2016). Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803. Plant and Cell Physiology, 57(1), 95-104. https://dx.doi.org/10.1093/pcp/pcv178 1471-9053 https://hdl.handle.net/10356/147060 10.1093/pcp/pcv178 26578692 2-s2.0-84964823183 1 57 95 104 en Plant and Cell Physiology © 2019 The Author(s). Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.This paper was published in Plant and Cell Physiology and is made available with permission of The Author(s). Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. application/pdf |
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Science Cyanobacteria Thylakoid Biogenesis Selão, Tiago Toscano Zhang, Lifang Knoppová, Jana Komenda, Josef Norling, Birgitta Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
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Thylakoid biogenesis is an intricate process requiring accurate and timely assembly of proteins, pigments and other cofactors into functional, photosynthetically competent membranes. PSII assembly is studied in particular as its core protein, D1, is very susceptible to photodamage and has a high turnover rate, particularly in high light. PSII assembly is a modular process, with assembly steps proceeding in a specific order. Using aqueous two-phase partitioning to separate plasma membranes (PM) and thylakoid membranes (TM), we studied the subcellular localization of the early assembly steps for PSII biogenesis in a Synechocystis sp. PCC6803 cyanobacterium strain lacking the CP47 antenna. This strain accumulates the early D1-D2 assembly complex which was localized in TM along with associated PSII assembly factors. We also followed insertion and processing of the D1 precursor (pD1) by radioactive pulse-chase labeling. D1 is inserted into the membrane with a C-terminal extension which requires cleavage by a specific protease, the C-terminal processing protease (CtpA), to allow subsequent assembly of the oxygen-evolving complex. pD1 insertion as well as its conversion to mature D1 under various light conditions was seen only in the TM. Epitope-tagged CtpA was also localized in the same membrane, providing further support for the thylakoid location of pD1 processing. However, Vipp1 and PratA, two proteins suggested to be part of the so-called 'thylakoid centers', were found to associate with the PM. Together, these results suggest that early PSII assembly steps occur in TM or specific areas derived from them, with interaction with PM needed for efficient PSII and thylakoid biogenesis. |
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School of Biological Sciences |
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School of Biological Sciences Selão, Tiago Toscano Zhang, Lifang Knoppová, Jana Komenda, Josef Norling, Birgitta |
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Article |
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Selão, Tiago Toscano Zhang, Lifang Knoppová, Jana Komenda, Josef Norling, Birgitta |
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Selão, Tiago Toscano |
title |
Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
title_short |
Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
title_full |
Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
title_fullStr |
Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
title_full_unstemmed |
Photosystem II assembly steps take place in the thylakoid membrane of the cyanobacterium Synechocystis sp. PCC6803 |
title_sort |
photosystem ii assembly steps take place in the thylakoid membrane of the cyanobacterium synechocystis sp. pcc6803 |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/147060 |
_version_ |
1759854059755405312 |