Tagging transferrin receptor with a disulfide FRET probe to gauge the redox state in endosomal compartments

• Main Authors: Bi, Xiaobao, Yin, Juan, Zhang, Dingpeng, Zhang, Xiaohong, Balamkundu, Seetharamsing, Lescar, Julien, Dedon, Peter C., Tam, James P., Liu, Chuan-Fa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science; Cell Membranes; Transferrin Receptors
• Online Access: https://hdl.handle.net/10356/148031
• Institution: Nanyang Technological University

Although the basic process of receptor-mediated endocytosis (RME) is well established, certain specific aspects, like the endosomal redox state, remain less characterized. Previous studies used chemically labeled ligands or antibodies with a FRET (fluorescence resonance energy transfer) probe to gau...