Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells

Bone tissue engineering has been a growing area of research due to an increase in bone diseases and fractures. Bone piezoelectricity, discovered by Fukuda and Yasuda, has led to the increasing interest in electrical stimulation for bone regeneration. Pulsed electromagnetic fields (PEMF) and piezoele...

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Main Author: Leong, Joshua Wei Ren
Other Authors: Swee Hin Teoh
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2021
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Online Access:https://hdl.handle.net/10356/148045
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spelling sg-ntu-dr.10356-1480452022-04-28T04:45:52Z Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells Leong, Joshua Wei Ren Swee Hin Teoh School of Chemical and Biomedical Engineering Dong YiBing teohsh@ntu.edu.sg Engineering::Bioengineering Bone tissue engineering has been a growing area of research due to an increase in bone diseases and fractures. Bone piezoelectricity, discovered by Fukuda and Yasuda, has led to the increasing interest in electrical stimulation for bone regeneration. Pulsed electromagnetic fields (PEMF) and piezoelectric material PVDF, were reported to positively impact on various stages of osteogenesis. Our previous study has shown the enhancement for bone regeneration using these two stimulations synergistically on 2 dimensional polycaprolactone-tricalciumphosphate (PCL-TCP) films. In this paper, the effects of PVDF coating under PEMF treatment of 0.6 mT, at 50 Hz frequency in 3D-printed PCL-TCP scaffolds, on the proliferation and mineralization of osteoblast precursor cells MC3T3-E1 were investiaged. Cell metabolic activity was assessed by alamarBlue time-course measurements and results indicated an increased in metabolic activity with electroactive material under PEMF exposure. Cell mineralization assessed by calcium deposition analysis at day 28, showed an increased in calcium with electroactive material with PEMF exposure. Alizarin Red staining was used to visualize the calcium deposition in the different groups and showed the highest calcium density for those groups with electroactive material. Quantitative polymerase chain reaction (qPCR) was done to monitor upregulation of genes corelated with osteoblastic differentiation and maturation. In summary, the results suggested that PEMF stimulation with electroactive material in 3D architecture could improve osteogenesis in vitro. Thus, this paper highlights the importance of selecting an electroactive scaffold material that would enhance the effect of PEMF on osteoblastic cells in a 3D environment. Bachelor of Engineering (Bioengineering) 2021-04-22T06:38:22Z 2021-04-22T06:38:22Z 2021 Final Year Project (FYP) Leong, J. W. R. (2021). Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/148045 https://hdl.handle.net/10356/148045 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Bioengineering
spellingShingle Engineering::Bioengineering
Leong, Joshua Wei Ren
Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
description Bone tissue engineering has been a growing area of research due to an increase in bone diseases and fractures. Bone piezoelectricity, discovered by Fukuda and Yasuda, has led to the increasing interest in electrical stimulation for bone regeneration. Pulsed electromagnetic fields (PEMF) and piezoelectric material PVDF, were reported to positively impact on various stages of osteogenesis. Our previous study has shown the enhancement for bone regeneration using these two stimulations synergistically on 2 dimensional polycaprolactone-tricalciumphosphate (PCL-TCP) films. In this paper, the effects of PVDF coating under PEMF treatment of 0.6 mT, at 50 Hz frequency in 3D-printed PCL-TCP scaffolds, on the proliferation and mineralization of osteoblast precursor cells MC3T3-E1 were investiaged. Cell metabolic activity was assessed by alamarBlue time-course measurements and results indicated an increased in metabolic activity with electroactive material under PEMF exposure. Cell mineralization assessed by calcium deposition analysis at day 28, showed an increased in calcium with electroactive material with PEMF exposure. Alizarin Red staining was used to visualize the calcium deposition in the different groups and showed the highest calcium density for those groups with electroactive material. Quantitative polymerase chain reaction (qPCR) was done to monitor upregulation of genes corelated with osteoblastic differentiation and maturation. In summary, the results suggested that PEMF stimulation with electroactive material in 3D architecture could improve osteogenesis in vitro. Thus, this paper highlights the importance of selecting an electroactive scaffold material that would enhance the effect of PEMF on osteoblastic cells in a 3D environment.
author2 Swee Hin Teoh
author_facet Swee Hin Teoh
Leong, Joshua Wei Ren
format Final Year Project
author Leong, Joshua Wei Ren
author_sort Leong, Joshua Wei Ren
title Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
title_short Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
title_full Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
title_fullStr Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
title_full_unstemmed Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
title_sort effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells
publisher Nanyang Technological University
publishDate 2021
url https://hdl.handle.net/10356/148045
_version_ 1734310280715829248