Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba

Background: The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biolo...

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Main Authors: Oropeza-Aburto, A., Cervantes-Pérez, S. A., Albert, Victor A., Herrera-Estrella, L.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/148369
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1483692023-02-28T17:08:47Z Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba Oropeza-Aburto, A. Cervantes-Pérez, S. A. Albert, Victor A. Herrera-Estrella, L. School of Biological Sciences Science::Biological sciences Agrobacterium Tumefaciens Genetic Transformation Background: The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologists since Darwin's early studies. Utricularia gibba is itself an aquatic plant with sophisticated bladder traps having one of the most complex suction mechanisms for trapping prey. However, the molecular characterization of the mechanisms that regulate trap development and the biophysical processes involved in prey trapping are still largely unknown due to the lack of a simple and reproducible gene transfer system. Results: Here, we report the establishment of a simple, fast and reproducible protocol for genetic transformation of U. gibba based on the T‑DNA of Agrobacterium tumefaciens. An in vitro selection system using Phosphinotricin as a selective agent was established for U. gibba. Plant transformation was confirmed by histochemical GUS assays and PCR and qRT‑PCR analyses. We report on the expression pattern of the 35S promoter and of the promoter of a trap‑specific ribonuclease gene in transgenic U. gibba plants. Conclusions: The genetic transformation protocol reported here is an effective method for studying developmental biology and functional genomics of this genus of carnivorous plants and advances the utility of U. gibba as a model system to study developmental processes involved in trap formation. Published version 2021-04-30T03:13:04Z 2021-04-30T03:13:04Z 2020 Journal Article Oropeza-Aburto, A., Cervantes-Pérez, S. A., Albert, V. A. & Herrera-Estrella, L. (2020). Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba. Plant Methods, 16(1). https://dx.doi.org/10.1186/s13007-020-00592-7 1746-4811 https://hdl.handle.net/10356/148369 10.1186/s13007-020-00592-7 32308728 2-s2.0-85083563610 1 16 en Plant Methods © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Agrobacterium Tumefaciens
Genetic Transformation
spellingShingle Science::Biological sciences
Agrobacterium Tumefaciens
Genetic Transformation
Oropeza-Aburto, A.
Cervantes-Pérez, S. A.
Albert, Victor A.
Herrera-Estrella, L.
Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
description Background: The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for carnivory is a feature of this genus that has been of great interest to biologists since Darwin's early studies. Utricularia gibba is itself an aquatic plant with sophisticated bladder traps having one of the most complex suction mechanisms for trapping prey. However, the molecular characterization of the mechanisms that regulate trap development and the biophysical processes involved in prey trapping are still largely unknown due to the lack of a simple and reproducible gene transfer system. Results: Here, we report the establishment of a simple, fast and reproducible protocol for genetic transformation of U. gibba based on the T‑DNA of Agrobacterium tumefaciens. An in vitro selection system using Phosphinotricin as a selective agent was established for U. gibba. Plant transformation was confirmed by histochemical GUS assays and PCR and qRT‑PCR analyses. We report on the expression pattern of the 35S promoter and of the promoter of a trap‑specific ribonuclease gene in transgenic U. gibba plants. Conclusions: The genetic transformation protocol reported here is an effective method for studying developmental biology and functional genomics of this genus of carnivorous plants and advances the utility of U. gibba as a model system to study developmental processes involved in trap formation.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Oropeza-Aburto, A.
Cervantes-Pérez, S. A.
Albert, Victor A.
Herrera-Estrella, L.
format Article
author Oropeza-Aburto, A.
Cervantes-Pérez, S. A.
Albert, Victor A.
Herrera-Estrella, L.
author_sort Oropeza-Aburto, A.
title Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
title_short Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
title_full Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
title_fullStr Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
title_full_unstemmed Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
title_sort agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant utricularia gibba
publishDate 2021
url https://hdl.handle.net/10356/148369
_version_ 1759853172104364032