Genome editing in mammalian cell lines using CRISPR-Cas

• Main Authors: Liu, Ivy Kaiwen, Norfala-Aliah Sutrisnoh, Wang, Yuanming, Tan, Meng How
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2021
• Subjects: Engineering::Chemical engineering; Genetics; Clustered Regularly Interspaced Short Palindromic Repeats
• Online Access: https://hdl.handle.net/10356/148681
• Institution: Nanyang Technological University

The clustered regularly interspaced short palindromic repeats (CRISPR) system functions naturally in bacterial adaptive immunity, but has been successfully repurposed for genome engineering in many different living organisms. Most commonly, the wildtype CRISPR associated 9 (Cas9) or Cas12a endonucle...