Effectiveness of mechanically filtered SDS on the decellularisation of porcine oesophagus

• Main Author: Don, Lee Jie
• Other Authors: Chian Kerm Sin
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2021
• Subjects: Engineering::Mechanical engineering
• Online Access: https://hdl.handle.net/10356/149124
• Institution: Nanyang Technological University

Extracellular matrix (ECM) for tissue engineering has been a research focus for tissue and organ regeneration. Oesophagus ECM are procured through the decellularisation process of porcine oesophagus. However, the process should retain its native folded structure and ECM scaffold properties to ensure proper regeneration and function of the tissue or organ minimising side-effects. The research was conducted on two different storage solutions of pre-decellularised porcine oesophagus (Phosphate-buffered saline with antibiotics and hydrogen peroxide 3%). Experiments were also conducted to determine the ideal decellularisation setup configuration to maximise both decellularisation efficiency and retainment of its native folded lumen structure. Recycling of waste sodium dodecyl sulfate (SDS) by mechanical filtration was carried out to determine the effect on decellularisation results. Results from this study shows that recycling of SDS is possible only with the combination of mechanical, chemical, and biological filters working together.