Study the effect of chelating agent on the decellularisation of oesophageal scaffold

• Main Author: Kwek, Nicholas Yi Wei
• Other Authors: Chian Kerm Sin
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2021
• Subjects: Engineering::Materials; Science::Biological sciences; Engineering::Mechanical engineering
• Online Access: https://hdl.handle.net/10356/149364
• Institution: Nanyang Technological University

Decellularising donor organs to obtain acellular scaffolds for the repair and regeneration of tissues and organs has shown encouraging results. The efficacies of a process ability to decellularize organs of animals are based on its ability to (1) preserve the mechanical integrity, (2) retain its extracellular matrix structures, and (3) complete removal of donor cells. In this project, 0.1 mM ethylenediamine tetra acetic acid and 0.2% sodium dodecyl sulphate were used to decellularize porcine oesophagi. The disinfecting effect of antibiotic-antimycotic and hydrogen peroxide on porcine oesophagi were also compared. Porcine oesophagi of full thickness were perfused with 0.1 mM ethylenediamine tetra acetic acid and 0.2% sodium dodecyl sulphate at 150 µl/min, varying treatment sequence and duration. Efficiency of the hybrid perfusion decellularisation treatment was assessed by characterising for residual DNA and histological analysis. Results showed that full thickness porcine oesophageal treated with the hybrid treatment showed promise in improving the efficacy of decellularisation while retaining its structural and ECM integrity. Although unable to achieve complete decellularisation, all variations of the hybrid treatment achieved on average, residual DNA content of 33% less than control setups and a significantly lower DNA content than native tissues. This study also showed that (1) 3% H2O2 has the potential to be an alternative solution for disinfecting and storing tissues and (2) to minimise radial expansion and preserve the plicated nature of the mucosa, perfusion pressure and duration must be kept under control.