Chemical modification of type 1 bullfrog collagen as cell encapsulant

• Main Author: Teh, Kar Yenn
• Other Authors: Dalton Tay Chor Yong
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2021
• Subjects: Engineering::Materials
• Online Access: https://hdl.handle.net/10356/149484
• Institution: Nanyang Technological University

Collagen is the most abundant protein in out body, due to its excellent biocompatibility, collagen had found its application in various biomedical and tissue engineering applications. Commercial collagen is commonly obtained from bovine or porcine sources. Recently, different sources of collagen are being explored, particularly from seafood waste that are commonly discarded as waste material such as skins and bones of fish and amphibian. In this study, we aim to extract and functionalise type-1 collagen derived from Bullfrog skin waste. The extraction will be carried out using mechanically assisted acid solubilised method. The extracted collagen will then undergo methacrylation and subsequently photocrosslinked to fabricate the collagen into hydrogel. The hydrogel will then be characterised based on its stability and cytocompatibility. At the end of study, a satisfactory yield of type-1 collagen with its triple helix structure preserved was successfully extracted from the source, the optimum extraction condition was also determined. The extracted collagen was successfully methacrylated by determining the optimum reaction parameters. Hydrogel from methacrylated collagen was also successfully fabricated and its formulation was optimized. The stability of hydrogel was determined and the hydrogel was found to be cytocompatibile. Overall, this study will present a new source of collagen derived from Bullfrog skin which is often discarded, transforming waste into valuable resources. This study also allows the application of collagen in the form of hydrogel by understanding its property.